MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
387 323.1486 322.1413
388 323.1486 322.1413
389 323.1487 322.1414
390 323.1487 322.1414
391 323.1494 322.1421
392 323.1671 322.1598
393 323.1674 322.1601
394 323.1678 322.1605
395 323.1841 322.1768
396 323.1842 322.1769
397 323.1844 322.1771
398 323.1848 322.1775
399 323.1848 322.1775
400 323.1852 322.1779
401 323.1852 322.1779
402 323.1853 322.1780
403 323.1855 322.1782
404 323.1855 322.1782
405 323.1855 322.1782
406 323.1855 322.1782
407 323.1855 322.1782
408 323.1857 322.1784
409 323.1858 322.1785
410 323.1862 322.1789
411 323.1864 322.1791
412 323.1866 322.1793
413 323.2051 322.1978
414 325.0888 324.0815
415 325.1260 324.1187
416 325.1264 324.1191
417 325.1266 324.1193
418 325.1268 324.1195
419 325.1270 324.1197
420 325.1272 324.1199
421 325.1272 324.1199
422 325.1275 324.1202
423 325.1277 324.1204
424 325.1278 324.1205
425 325.1284 324.1211
426 325.1632 324.1559
427 325.1635 324.1562
428 325.1639 324.1566
429 325.1642 324.1569
430 325.1646 324.1573
431 325.1826 324.1753
432 325.1826 324.1753
433 325.1829 324.1756
434 325.1830 324.1757
435 325.2212 324.2139
436 326.0454 325.0381
437 326.0460 325.0387
438 326.0463 325.0390
439 326.0464 325.0391
440 326.0464 325.0391
441 326.0468 325.0395
442 326.1833 325.1760
443 326.1834 325.1761
444 326.1967 325.1894
445 326.1971 325.1898
446 326.1981 325.1908
447 327.0760 326.0687
448 327.0767 326.0694
449 327.1049 326.0976
450 327.1053 326.0980
451 327.1053 326.0980
452 327.1053 326.0980
453 327.1432 326.1359
454 327.1432 326.1359
455 327.1433 326.1360
456 327.1441 326.1368
457 327.1443 326.1370
458 327.1446 326.1373
459 327.1786 326.1713
460 327.1788 326.1715
461 327.1795 326.1722
462 327.1995 326.1922
463 327.2005 326.1932
464 327.2007 326.1934
465 327.6650 326.6577
466 327.8613 326.8540
467 328.0169 327.0096
468 328.0173 327.0100
469 328.0259 327.0186
470 328.0262 327.0189
471 328.1565 327.1492
472 328.2138 327.2065
473 329.0013 327.9940
474 329.0020 327.9947
475 329.0022 327.9949
476 329.0025 327.9952
477 329.0035 327.9962
478 329.0549 328.0476
479 329.0919 328.0846
480 329.1237 328.1164
481 329.1578 328.1505
482 329.1581 328.1508
483 329.1584 328.1511
484 329.1587 328.1514
485 329.1591 328.1518
486 329.1591 328.1518
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