MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 3989)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1568 545.3411 544.3338 544.3333 1.00 0 22 0.064 +1Score > 23 indicates identity VIASR
1569 545.3441 544.3368 544.3333 6.51 0 22 0.072 +1Score > 23 indicates identity
Score > 23 indicates homology		 VIASR
1643 617.3259 616.3186 616.3180 1.00 0 20 0.074 +1Score > 21 indicates identity EEAIR
1524 503.2936 502.2863 502.2863 -0.014 0 18 0.088 +1Score > 22 indicates identity
Score > 20 indicates homology		 ISGAR
1887 802.4577 801.4504 801.4344 20.0 1 18 0.1 +1Score > 20 indicates identity EKVEAAR
1526 503.2973 502.2900 502.2863 7.35 0 17 0.11 +1Score > 22 indicates identity
Score > 20 indicates homology		 ISGAR
1556 533.2571 532.2498 532.2493 1.04 0 17 0.062 +1Score > 17 indicates identity EEAGK
1886 401.7317 801.4488 801.4596 -13.4 0 17 0.11 +1Score > 20 indicates identity
Score > 19 indicates homology		 VSAVIDAK
2510 406.5370 1216.5892 1216.6047 -12.8 1 16 0.098 +1Score > 19 indicates identity QTRNALADAQK + 2 Deamidated (NQ)
1888 802.4609 801.4536 801.4596 -7.47 0 16 0.087 +1Score > 18 indicates identity VSAVIDAK
1717 674.3519 673.3446 673.3395 7.62 0 16 0.14 +1Score > 20 indicates identity LDGVNR + Deamidated (NQ)
2050 472.2621 942.5096 942.5246 -15.9 1 16 0.068 +1Score > 17 indicates identity IALAENRR + Deamidated (NQ)
2383 568.7909 1135.5672 1135.5509 14.4 0 16 0.14 +1Score > 20 indicates identity NFLAETGDIR + Deamidated (NQ)
1571 547.2483 546.2410 546.2398 2.28 0 16 0.066 +1Score > 16 indicates identity DAEGR
2445 586.3212 1170.6278 1170.6244 2.92 0 16 0.11 +1Score > 18 indicates identity QQAQVEQVLK + Deamidated (NQ)
1830 380.2169 758.4192 758.4286 -12.4 0 15 0.17 +1Score > 20 indicates identity IIETQR
1831 380.2170 758.4194 758.4286 -12.1 0 15 0.16 +1Score > 20 indicates identity
Score > 20 indicates homology		 IIETQR
1930 831.4260 830.4187 830.4246 -7.07 1 15 0.15 +1Score > 20 indicates identity DINNKAR + Deamidated (NQ)
2556 626.3234 1250.6322 1250.6367 -3.56 0 15 0.15 +1Score > 19 indicates identity ANQLAELAHQR + Deamidated (NQ)
1736 687.4149 686.4076 686.4075 0.18 0 15 0.18 +1Score > 20 indicates identity AITINR
2012 914.4950 913.4877 913.5055 -19.5 0 14 0.074 +1Score > 16 indicates identity NGLMPLIR + Deamidated (NQ)
2364 563.8127 1125.6108 1125.6295 -16.5 0 14 0.085 +1Score > 16 indicates identity IALQLFHAGR + Deamidated (NQ)
2065 474.7336 947.4526 947.4672 -15.4 1 14 0.12 +1Score > 18 indicates identity DGKVTSDAR
1799 731.4202 730.4129 730.4126 0.47 1 14 0.18 +1Score > 19 indicates identity EWIKR
2236 514.2934 1026.5722 1026.5822 -9.66 0 14 0.082 +1Score > 16 indicates identity LLEQQQLR
1642 616.3798 615.3725 615.3704 3.46 0 14 0.21 +1Score > 21 indicates identity
Score > 20 indicates homology		 IIQSR
2336 557.8105 1113.6064 1113.6142 -6.94 1 14 0.17 +1Score > 19 indicates identity LAQLADEAKR
1557 533.2591 532.2518 532.2493 4.80 0 14 0.2 +1Score > 19 indicates identity EEAGK
2854 520.6209 1558.8409 1558.8389 1.30 1 14 0.12 +1Score > 17 indicates identity ITLNMGVGEAIADKK
1679 650.2953 649.2880 649.2853 4.14 0 14 0.06 +1Score > 14 indicates identity EQAMR + Oxidation (M)
1955 428.7679 855.5212 855.5290 -9.05 1 14 0.086 +1Score > 16 indicates identity IANRLLR + Deamidated (NQ)
2146 491.2510 980.4874 980.5039 -16.8 1 14 0.13 +1Score > 17 indicates identity QQRYSATK
2528 614.8384 1227.6622 1227.6823 -16.3 0 14 0.19 +1Score > 19 indicates identity QSLNSLLSPLR + Deamidated (NQ)
1774 358.2086 714.4026 714.4024 0.34 0 13 0.21 +1Score > 19 indicates identity QNALLR + Deamidated (NQ)
1974 873.4944 872.4871 872.4716 17.8 0 13 0.26 +1Score > 20 indicates identity QLEGITGR
1836 761.3802 760.3729 760.3715 1.83 0 13 0.27 +1Score > 19 indicates identity SDSPVTR
1779 359.1811 716.3476 716.3453 3.27 0 12 0.18 +1Score > 18 indicates identity GQDIER
1823 377.1796 752.3446 752.3340 14.1 0 12 0.13 +1Score > 16 indicates identity AGNEYAK + Deamidated (NQ)
2337 557.8115 1113.6084 1113.6142 -5.15 1 12 0.26 +1Score > 18 indicates identity QALELVRER + Deamidated (NQ)
2241 517.7869 1033.5592 1033.5556 3.50 0 12 0.22 +1Score > 18 indicates identity LAQGEFLTR
1885 401.7230 801.4314 801.4457 -17.8 1 12 0.33 +1Score > 20 indicates identity TVAEARR
2938 536.5743 1606.7011 1606.7256 -15.3 1 12 0.098 +1Score > 14 indicates identity QTQLTEEMRQNAR + 3 Deamidated (NQ)
1737 344.2111 686.4076 686.4075 0.24 1 12 0.36 +1Score > 20 indicates identity AIKEAR
1941 422.2723 842.5300 842.5225 8.91 1 12 0.089 +1Score > 14 indicates identity LDLVQKK
1820 373.6945 745.3744 745.3719 3.48 0 12 0.35 +1Score > 20 indicates identity ELSGGQR
1837 761.3812 760.3739 760.3715 3.14 0 12 0.35 +1Score > 19 indicates identity SDSPVTR
1740 344.2131 686.4116 686.4075 6.06 1 11 0.21 +1Score > 17 indicates identity AIKEAR
1678 650.2947 649.2874 649.2853 3.21 0 11 0.098 +1Score > 14 indicates identity EQAMR + Oxidation (M)
2423 578.3441 1154.6736 1154.6546 16.5 1 11 0.14 +1Score > 15 indicates identity EPEKVALLQK + Deamidated (NQ)
2526 614.8351 1227.6556 1227.6571 -1.19 1 11 0.29 +1Score > 18 indicates identity QRLDAINQLR + 2 Deamidated (NQ)
1641 308.6927 615.3708 615.3704 0.74 0 11 0.5 +1Score > 22 indicates identity
Score > 21 indicates homology		 IIQSR
1858 789.4289 788.4216 788.4141 9.59 0 11 0.32 +1Score > 19 indicates identity TQSQAVR
2184 332.4931 994.4575 994.4720 -14.6 0 11 0.22 +1Score > 17 indicates identity QEGAFSLSR + Deamidated (NQ)
1719 675.3416 674.3343 674.3348 -0.65 0 11 0.35 +1Score > 19 indicates identity DVATGGR
1975 873.4982 872.4909 872.4967 -6.64 0 11 0.38 +1Score > 19 indicates identity EQGLLSVK
1720 675.3421 674.3348 674.3348 0.090 0 11 0.37 +1Score > 19 indicates identity DVATGGR
2004 455.2625 908.5104 908.4967 15.1 0 11 0.11 +1Score > 14 indicates identity IAVSYQTK
1721 675.3469 674.3396 674.3348 7.21 0 11 0.33 +1Score > 19 indicates identity DVATGGR
4256 686.3244 3426.5856 3426.6316 -13.4 1 11 0.26 +1Score > 17 indicates identity NLQTTLDSMNKILASQSMQQLPTDMQSTLR + 2 Deamidated (NQ); 2 Oxidation (M)
2397 571.8590 1141.7034 1141.7070 -3.14 1 11 0.088 +1Score > 13 indicates identity KQTALVELLK
1872 401.2321 800.4496 800.4392 13.1 0 10 0.46 +1Score > 20 indicates identity SINPTIR + Deamidated (NQ)
2940 804.8845 1607.7544 1607.7831 -17.8 1 10 0.4 +1Score > 20 indicates identity
Score > 19 indicates homology		 AWFKLDEIQQSDK + Deamidated (NQ)
1835 381.1902 760.3658 760.3616 5.56 1 10 0.37 +1Score > 19 indicates identity QRDWR + Deamidated (NQ)
1914 412.6981 823.3816 823.3824 -0.95 0 10 0.16 +1Score > 15 indicates identity VSAGYGDR
3502 557.5233 2226.0641 2226.0586 2.46 1 10 0.47 +1Score > 19 indicates identity KMHGINSVPGGVNNNLSIAER + 4 Deamidated (NQ); Oxidation (M)
2532 616.7827 1231.5508 1231.5680 -13.9 0 10 0.17 +1Score > 15 indicates identity TDAEQAQELAR + Deamidated (NQ)
3571 1152.0632 2302.1118 2302.0722 17.2 1 10 0.5 +1Score > 19 indicates identity FATEGMMQVLADEYQQRLR + Deamidated (NQ); Oxidation (M)
1730 342.6561 683.2976 683.2987 -1.50 0 10 0.11 +1Score > 13 indicates identity HQNER + Deamidated (NQ)
1801 731.4230 730.4157 730.4086 9.82 1 10 0.47 +1Score > 19 indicates identity ERISAR
2014 914.4968 913.4895 913.5055 -17.5 0 10 0.21 +1Score > 16 indicates identity NGLMPLIR + Deamidated (NQ)
2069 474.7361 947.4576 947.4712 -14.3 0 10 0.37 +1Score > 18 indicates identity DGNIFAPSK
2310 547.7496 1093.4846 1093.5040 -17.7 0 10 0.28 +1Score > 17 indicates identity IADGYEQAAR + Deamidated (NQ)
2722 714.3479 1426.6812 1426.6800 0.85 0 10 0.43 +1Score > 18 indicates identity SSLNQNTEHQLR + Deamidated (NQ)
2351 560.8027 1119.5908 1119.5706 18.1 1 10 0.43 +1Score > 18 indicates identity RSQSMELLR + Deamidated (NQ)
3186 922.9919 1843.9692 1843.9502 10.3 0 10 0.14 +1Score > 20 indicates identity
Score > 13 indicates homology		 ITPHIISQIMEFAATR + Deamidated (NQ); Oxidation (M)
2309 547.7491 1093.4836 1093.5040 -18.6 0 9 0.31 +1Score > 17 indicates identity IADGYEQAAR + Deamidated (NQ)
1622 302.1771 602.3396 602.3388 1.47 0 9 0.51 +1Score > 19 indicates identity ISDIR
1915 824.3906 823.3833 823.3824 1.08 0 9 0.19 +1Score > 15 indicates identity VSAGYGDR
2201 501.7908 1001.5670 1001.5506 16.5 0 9 0.49 +1Score > 19 indicates identity SGETVALLGR
1570 545.3449 544.3376 544.3333 7.98 0 9 0.45 +1Score > 23 indicates identity
Score > 18 indicates homology		 ASVIR
2292 536.7865 1071.5584 1071.5713 -12.0 0 9 0.56 -1Score > 19 indicates identity DIWQLQLR + Deamidated (NQ)
2721 357.6775 1426.6809 1426.6800 0.60 0 9 0.48 +1Score > 18 indicates identity SSLNQNTEHQLR + Deamidated (NQ)
2723 714.3484 1426.6822 1426.6800 1.55 0 9 0.48 +1Score > 18 indicates identity SSLNQNTEHQLR + Deamidated (NQ)
2450 588.2912 1174.5678 1174.5877 -16.9 1 9 0.6 +1Score > 19 indicates identity QGGRTMEIQR
1654 625.2949 624.2876 624.2867 1.44 0 9 0.31 +1Score > 16 indicates identity DAYTR
1328 417.2470 416.2397 416.2383 3.39 0 9 0.58 +1Score > 25 indicates identity
Score > 19 indicates homology		 AGAAK
1927 415.7368 829.4590 829.4545 5.47 0 9 0.74 +1Score > 20 indicates identity QNDILVK + Deamidated (NQ)
1995 449.7614 897.5082 897.5144 -6.88 1 9 0.17 +1Score > 14 indicates identity LAGSPRAAR
3133 898.4305 1794.8464 1794.8240 12.5 0 9 0.57 +1Score > 19 indicates identity SLCQSMIDNQQLVSR + Deamidated (NQ); Oxidation (M)
2125 485.7090 969.4034 969.4226 -19.7 0 9 0.13 +1Score > 13 indicates identity GAAMEGQYK + Oxidation (M)
1602 575.3173 574.3100 574.3075 4.46 0 9 0.58 +1Score > 21 indicates identity
Score > 19 indicates homology		 EGTLR
1931 833.4194 832.4121 832.4079 5.06 0 9 0.56 +1Score > 19 indicates identity FLPDGQR + Deamidated (NQ)
2627 449.5523 1345.6351 1345.6109 17.9 0 9 0.4 +1Score > 17 indicates identity LNQVEQSNQQR + 3 Deamidated (NQ)
1558 533.2745 532.2672 532.2605 12.6 0 9 0.48 +1Score > 18 indicates identity QGTAR + Deamidated (NQ)
2492 401.8763 1202.6071 1202.5891 15.0 1 9 0.19 +1Score > 20 indicates identity
Score > 14 indicates homology		 NAQQIADRASK + 2 Deamidated (NQ)
2517 611.8100 1221.6054 1221.5845 17.1 1 9 0.6 +1Score > 19 indicates identity KLLNQAMACR + 2 Deamidated (NQ); Oxidation (M)
1857 395.2170 788.4194 788.4181 1.76 0 9 0.58 +1Score > 19 indicates identity WLNISR + Deamidated (NQ)
3370 408.4018 2036.9726 2036.9836 -5.41 1 9 0.67 +1Score > 19 indicates identity APATQEEISEYNKLMASR
3340 503.0111 2008.0153 2008.0014 6.93 0 9 0.65 +1Score > 19 indicates identity KPFEGNLNAGYLAQSGNTK
2260 522.2999 1042.5852 1042.5659 18.6 0 8 0.65 +1Score > 19 indicates identity AVVGQQEAIK + Deamidated (NQ)
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