MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:35:06 GMT |
Not what you expected? Try 
the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RHAA_ECOLI | 6577 | L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PEPB_ECOLI | 1618 | Peptidase B OS=Escherichia coli (strain K12) GN=pepB PE=3 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IADA_ECOLI | 651 | Isoaspartyl dipeptidase OS=Escherichia coli (strain K12) GN=iadA PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CODA_ECOLI | 463 | Cytosine deaminase OS=Escherichia coli (strain K12) GN=codA PE=1 SV=3 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ALF1_ECOLI | 333 | Fructose-bisphosphate aldolase class 1 OS=Escherichia coli (strain K12) GN=fbaB PE=1 SV=2 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CATNterm_ECOLX | 324 | Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YGEY_ECOLI | 280 | Uncharacterized protein YgeY OS=Escherichia coli (strain K12) GN=ygeY PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KDSC_ECOLI | 270 | 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC OS=Escherichia coli (strain K12) GN=kdsC PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 8.1 | KDSC_ECOLI |
270 | 20213 | 16 (10) | 10 (6) | 1.94 | |
| 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC OS=Escherichia coli (strain K12) GN=kdsC PE=1 SV=1 | |||||||
16 peptide matches (11 non-duplicate, 5 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 1619 |
1 |
301.6679 | 601.3212 | 601.3183 | 4.83 | 0 | 20 | 0.062 | 1Score > 20 indicates identity |
U | K.AENIR.L |
| 1624 |
303.6718 | 605.3290 | 605.3285 | 0.84 | 0 | 12 | 0.16 | 1Score > 17 indicates identity |
U | K.AFNVR.D | |
| 1657 |
1 |
316.1751 | 630.3356 | 630.3337 | 3.12 | 0 | 37 | 0.0012 | 1Score > 20 indicates identity |
U | K.LVEDR.C |
| 1786 |
1 |
362.6855 | 723.3564 | 723.3551 | 1.80 | 0 | 30 | 0.003 | 1Score > 17 indicates identity |
U | R.ADYVTR.I |
| 2411 |
1 |
574.8360 | 1147.6574 | 1147.6488 | 7.49 | 0 | 55 | 5.8e-006 | 1Score > 15 indicates identity |
U | K.LIAFSDLLEK.L |
| 2641 |
679.8733 | 1357.7320 | 1357.7275 | 3.33 | 0 | 44 | 0.00015 | 1Score > 18 indicates identity |
U | R.EVCDLLLLAQGK.L | |
| 3089 |
1 |
576.6794 | 1727.0164 | 1727.0094 | 4.05 | 0 | 67 | 1.8e-007 | 1Score > 13 indicates identity |
U | K.VGLSVAVADAHPLLIPR.A |
D:\Xcalibur\Data\Jennifer\PRT1258 ThomasE\PRT1258_JBEI_Tube2_A2.rawScore > 13 indicates identity |
|||||||||||
| 3092 |
864.5208 | 1727.0270 | 1727.0094 | 10.2 | 0 | 59 | 1.1e-006 | 1Score > 13 indicates identity |
U | K.VGLSVAVADAHPLLIPR.A | |
| No other peptide matches in query | |||||||||||
| 3125 |
597.9689 | 1790.8849 | 1790.8621 | 12.7 | 0 | 4 | 1 | 1Score > 20 indicates identity Score > 16 indicates homology |
U | R.CATLGITHLYQGQSNK.L + Deamidated (NQ) | |
| 3771 |
859.1089 | 2574.3049 | 2574.2563 | 18.9 | 0 | 24 | 0.018 | 1Score > 19 indicates identity |
U | K.LAIAPENVAYVGDDLIDWPVMEK.V + Deamidated (NQ); Oxidation (M) | |
| 3877 |
903.1434 | 2706.4084 | 2706.3673 | 15.2 | 0 | 17 | 0.063 | 1Score > 18 indicates identity |
U | R.LLILDVDGVLSDGLIYMGNNGEELK.A + Deamidated (NQ); Oxidation (M) | |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| UPI0002C322D5 |
37 | 0 | 8.1 | ||
| description | |||||
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 263 | Trypsin OS=Sus scrofa PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLDA_ECOLI | 261 | Glycerol dehydrogenase OS=Escherichia coli (strain K12) GN=gldA PE=1 SV=1 |
Not what you expected? Try the select summary.
Mascot: http://www.matrixscience.com/