MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 63)

Page: 1 2 3 4 5 6 … 7 Next 

+1

Accession Score Description
1 RHAA_ECOLI 6577 L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2

+2

Accession Score Description
1 PEPB_ECOLI 1618 Peptidase B OS=Escherichia coli (strain K12) GN=pepB PE=3 SV=2

+3

Accession Score Description
1 IADA_ECOLI 651 Isoaspartyl dipeptidase OS=Escherichia coli (strain K12) GN=iadA PE=1 SV=1

+4

Accession Score Description
1 CODA_ECOLI 463 Cytosine deaminase OS=Escherichia coli (strain K12) GN=codA PE=1 SV=3

+5

Accession Score Description
1 ALF1_ECOLI 333 Fructose-bisphosphate aldolase class 1 OS=Escherichia coli (strain K12) GN=fbaB PE=1 SV=2

+6

Accession Score Description
1 CATNterm_ECOLX 324 Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1

+7

Accession Score Description
1 YGEY_ECOLI 280 Uncharacterized protein YgeY OS=Escherichia coli (strain K12) GN=ygeY PE=3 SV=1

-8

Accession Score Description
1 KDSC_ECOLI 270 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC OS=Escherichia coli (strain K12) GN=kdsC PE=1 SV=1
Score Mass Matches Sequences emPAI
8.1 KDSC_ECOLI 270 20213 16 (10) 10 (6) 1.94
3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC OS=Escherichia coli (strain K12) GN=kdsC PE=1 SV=1

-16 peptide matches (11 non-duplicate, 5 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1619 +1 301.6679 601.3212 601.3183 4.83 0 20 0.062 +1Score > 20 indicates identity U K.AENIR.L
1624   303.6718 605.3290 605.3285 0.84 0 12 0.16 +1Score > 17 indicates identity U K.AFNVR.D
D:\Xcalibur\Data\Jennifer\PRT1258 ThomasE\PRT1258_JBEI_Tube2_A2.raw

Score > 20 indicates identity
1657 +1 316.1751 630.3356 630.3337 3.12 0 37 0.0012 -1Score > 20 indicates identity U K.LVEDR.C
3.12 0 37 0.0012 1 LVQDR   + Deamidated (NQ)
3.12 0 19 0.076 3 VIQDR   + Deamidated (NQ)
3.17 0 19 0.076 4 INLNR   + 2 Deamidated (NQ)
3.12 0 19 0.076 4 LDLDR  
3.15 0 19 0.076 4 LEVNR   + Deamidated (NQ)
3.15 0 19 0.076 4 LINDR   + Deamidated (NQ)
3.15 0 19 0.076 4 LNLDR   + Deamidated (NQ)
3.12 0 10 0.64 9 IDDIR  
3.17 0 10 0.64 9 LNNLR   + 2 Deamidated (NQ)
1786 +1 362.6855 723.3564 723.3551 1.80 0 30 0.003 +1Score > 17 indicates identity U R.ADYVTR.I
2411 +1 574.8360 1147.6574 1147.6488 7.49 0 55 5.8e-006 +1Score > 15 indicates identity U K.LIAFSDLLEK.L
2641   679.8733 1357.7320 1357.7275 3.33 0 44 0.00015 +1Score > 18 indicates identity U R.EVCDLLLLAQGK.L
3089 +1 576.6794 1727.0164 1727.0094 4.05 0 67 1.8e-007 +1Score > 13 indicates identity U K.VGLSVAVADAHPLLIPR.A
3092   864.5208 1727.0270 1727.0094 10.2 0 59 1.1e-006 +1Score > 13 indicates identity U K.VGLSVAVADAHPLLIPR.A
3125   597.9689 1790.8849 1790.8621 12.7 0 4 1 +1Score > 20 indicates identity
Score > 16 indicates homology		 U R.CATLGITHLYQGQSNK.L + Deamidated (NQ)
3771   859.1089 2574.3049 2574.2563 18.9 0 24 0.018 +1Score > 19 indicates identity U K.LAIAPENVAYVGDDLIDWPVMEK.V + Deamidated (NQ); Oxidation (M)
3877   903.1434 2706.4084 2706.3673 15.2 0 17 0.063 +1Score > 18 indicates identity U R.LLILDVDGVLSDGLIYMGNNGEELK.A + Deamidated (NQ); Oxidation (M)

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
UPI0002C322D5 37 0 8.1
description

+9

Accession Score Description
1 TRYP_PIG 263 Trypsin OS=Sus scrofa PE=1 SV=1

+10

Accession Score Description
1 GLDA_ECOLI 261 Glycerol dehydrogenase OS=Escherichia coli (strain K12) GN=gldA PE=1 SV=1
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