MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:35:06 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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61| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | BFR_ECOLI | 19 | Bacterioferritin OS=Escherichia coli (strain K12) GN=bfr PE=1 SV=1 |
62| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KRT83_HUMAN | 16 | Keratin, type II cuticular Hb3 OS=Homo sapiens GN=KRT83 PE=1 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 62.1 | KRT83_HUMAN |
16 | 55928 | 2 (1) | 2 (1) | 0.06 | |
| Keratin, type II cuticular Hb3 OS=Homo sapiens GN=KRT83 PE=1 SV=2 | |||||||
| 2 samesets of KRT83_HUMAN | |||||||
| KRT81_HUMAN |
16 | 56832 | 1 (1) | 1 (1) | 0.06 | ||
| Keratin, type II cuticular Hb1 OS=Homo sapiens GN=KRT81 PE=1 SV=3 | |||||||
| KRT86_HUMAN |
16 | 55120 | 1 (1) | 1 (1) | 0.06 | ||
| Keratin, type II cuticular Hb6 OS=Homo sapiens GN=KRT86 PE=1 SV=1 | |||||||
2 peptide matches (2 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2207 |
503.7364 | 1005.4582 | 1005.4736 | -15.2 | 0 | 16 | 0.058 | 1Score > 16 indicates identity |
U | K.QDMACLIR.E | |
| 2383 |
568.7909 | 1135.5672 | 1135.5873 | -17.7 | 1 | 9 | 0.68 | 4Score > 20 indicates identity |
K.KYEEEVALR.A |
63| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | POTD_ECOLI | 15 | Spermidine/putrescine-binding periplasmic protein OS=Escherichia coli (strain K12) GN=potD PE=1 SV=1 |
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