MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:35:06 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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51| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HEMN_ECOLI | 27 | Oxygen-independent coproporphyrinogen-III oxidase OS=Escherichia coli (strain K12) GN=hemN PE=1 SV=4 |
52| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | BIOD2_ECOLI | 26 | ATP-dependent dethiobiotin synthetase BioD 2 OS=Escherichia coli (strain K12) GN=bioD2 PE=3 SV=1 |
53| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NUDK_ECOLI | 25 | GDP-mannose pyrophosphatase NudK OS=Escherichia coli (strain K12) GN=nudK PE=1 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 53.1 | NUDK_ECOLI |
25 | 21849 | 6 (1) | 2 (1) | 0.15 | |
| GDP-mannose pyrophosphatase NudK OS=Escherichia coli (strain K12) GN=nudK PE=1 SV=2 | |||||||
| 3 samesets of NUDK_ECOLI | |||||||
| RECQ_ECOLI |
25 | 0 | 4 (1) | 2 (1) | 0.05 | ||
| description | |||||||
| XDHD_ECOLI |
25 | 104366 | 6 (1) | 2 (1) | 0.03 | ||
| Probable hypoxanthine oxidase XdhD OS=Escherichia coli (strain K12) GN=xdhD PE=3 SV=1 | |||||||
| YHDX_ECOLI |
25 | 0 | 3 (1) | 1 (1) | 0.08 | ||
| description | |||||||
6 peptide matches (2 non-duplicate, 4 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1601 |
4 |
575.3168 | 574.3095 | 574.3075 | 3.59 | 0 | 25 | 0.019 | 1Score > 21 indicates identity |
U | K.TGEIR.D |
| 2307 |
546.8101 | 1091.6056 | 1091.5896 | 14.7 | 0 | 15 | 0.085 | 1Score > 17 indicates identity |
U | -.MTQQITLIK.D + Deamidated (NQ); Oxidation (M) |
54| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EPTA_ECOLI | 24 | Phosphoethanolamine transferase EptA OS=Escherichia coli (strain K12) GN=eptA PE=1 SV=2 |
55| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YJCE_ECOLI | 23 | Uncharacterized Na(+)/H(+) exchanger YjcE OS=Escherichia coli (strain K12) GN=yjcE PE=3 SV=1 |
56| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YEGI_ECOLI | 22 | Uncharacterized protein YegI OS=Escherichia coli (strain K12) GN=yegI PE=4 SV=1 |
57| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FABF_ECOLI | 22 | 3-oxoacyl-[acyl-carrier-protein] synthase 2 OS=Escherichia coli (strain K12) GN=fabF PE=1 SV=2 |
58| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PYRD_ECOLI | 20 | Dihydroorotate dehydrogenase (quinone) OS=Escherichia coli (strain K12) GN=pyrD PE=1 SV=1 |
59| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YQIG_ECOLI | 20 | description |
60| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PSTS_ECOLI | 20 | Phosphate-binding protein PstS OS=Escherichia coli (strain K12) GN=pstS PE=1 SV=1 |
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