MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:35:06 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RHAA_ECOLI | 6577 | L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PEPB_ECOLI | 1618 | Peptidase B OS=Escherichia coli (strain K12) GN=pepB PE=3 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IADA_ECOLI | 651 | Isoaspartyl dipeptidase OS=Escherichia coli (strain K12) GN=iadA PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CODA_ECOLI | 463 | Cytosine deaminase OS=Escherichia coli (strain K12) GN=codA PE=1 SV=3 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ALF1_ECOLI | 333 | Fructose-bisphosphate aldolase class 1 OS=Escherichia coli (strain K12) GN=fbaB PE=1 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 5.1 | ALF1_ECOLI |
333 | 38313 | 13 (13) | 10 (10) | 1.49 | |
| Fructose-bisphosphate aldolase class 1 OS=Escherichia coli (strain K12) GN=fbaB PE=1 SV=2 | |||||||
13 peptide matches (11 non-duplicate, 2 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 1777 |
1 |
358.7397 | 715.4648 | 715.4632 | 2.24 | 0 | 27 | 0.0019 | 1Score > 13 indicates identity |
U | R.IPFLVK.L |
D:\Xcalibur\Data\Jennifer\PRT1258 ThomasE\PRT1258_JBEI_Tube2_A2.rawScore > 17 indicates identity |
|||||||||||
| 1873 |
401.2524 | 800.4902 | 800.4868 | 4.29 | 1 | 19 | 0.036 | 1Score > 17 indicates identity |
U | R.TAVINKR.A | |
| No other peptide matches in query | |||||||||||
| 2299 |
361.1864 | 1080.5374 | 1080.5312 | 5.71 | 0 | 25 | 0.0078 | 1Score > 17 indicates identity |
U | K.DADNLLQHR.C | |
| 2300 |
541.2767 | 1080.5388 | 1080.5312 | 7.07 | 0 | 30 | 0.0028 | 1Score > 17 indicates identity |
U | K.DADNLLQHR.C | |
| 2563 |
1 |
628.8468 | 1255.6790 | 1255.6772 | 1.47 | 0 | 59 | 5.1e-006 | 1Score > 18 indicates identity |
U | K.LTSENPIDLVR.Y |
| 2590 |
646.8290 | 1291.6434 | 1291.6408 | 2.06 | 0 | 41 | 0.00032 | 1Score > 19 indicates identity |
U | R.QIEEISAAFER.A | |
| 2789 |
739.4037 | 1476.7928 | 1476.7824 | 7.08 | 0 | 70 | 2.9e-007 | 1Score > 17 indicates identity |
U | K.LINAVQDVYLDSK.I | |
| 2824 |
504.2800 | 1509.8182 | 1509.8086 | 6.36 | 0 | 17 | 0.047 | 1Score > 16 indicates identity |
U | R.VMIDNNRPPAVLR.N + Oxidation (M) | |
| 3038 |
555.6050 | 1663.7932 | 1663.7842 | 5.40 | 1 | 27 | 0.008 | 1Score > 18 indicates identity |
U | K.AINYGYTDDRVYSK.L | |
| 3263 |
965.9786 | 1929.9426 | 1929.9392 | 1.79 | 0 | 100 | 5.7e-010 | 1Score > 20 indicates identity |
U | R.AGLINSGGAAGGETDLSDAVR.T | |
| 4278 |
1169.6089 | 3505.8049 | 3505.7405 | 18.4 | 0 | 52 | 1.4e-005 | 1Score > 16 indicates identity |
U | R.LAGTGYLSILPVDQGVEHSAGASFAANPLYFDPK.N + Deamidated (NQ) | |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CATNterm_ECOLX | 324 | Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YGEY_ECOLI | 280 | Uncharacterized protein YgeY OS=Escherichia coli (strain K12) GN=ygeY PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KDSC_ECOLI | 270 | 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC OS=Escherichia coli (strain K12) GN=kdsC PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 263 | Trypsin OS=Sus scrofa PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLDA_ECOLI | 261 | Glycerol dehydrogenase OS=Escherichia coli (strain K12) GN=gldA PE=1 SV=1 |
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