MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:35:06 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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41| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SODF_ECOLI | 38 | Superoxide dismutase [Fe] OS=Escherichia coli (strain K12) GN=sodB PE=1 SV=2 |
42| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YJJJ_ECOLI | 36 | Putative kinase YjjJ OS=Escherichia coli (strain K12) GN=yjjJ PE=3 SV=1 |
43| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SGCX_ECOLI | 35 | Putative aminopeptidase SgcX OS=Escherichia coli (strain K12) GN=sgcX PE=3 SV=3 |
44| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TOLC_ECOLI | 34 | Outer membrane protein TolC OS=Escherichia coli (strain K12) GN=tolC PE=1 SV=3 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 44.1 | TOLC_ECOLI |
34 | 53708 | 5 (2) | 5 (2) | 0.13 | |
| Outer membrane protein TolC OS=Escherichia coli (strain K12) GN=tolC PE=1 SV=3 | |||||||
5 peptide matches (5 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1885 |
401.7230 | 801.4314 | 801.4344 | -3.74 | 0 | 10 | 0.45 | 2Score > 20 indicates identity |
U | R.LSQDLAR.E | |
| 1888 |
802.4609 | 801.4536 | 801.4596 | -7.45 | 0 | 3 | 1.8 | 8Score > 18 indicates identity |
U | R.ALTLQEK.A | |
| 2087 |
319.5230 | 955.5472 | 955.5450 | 2.23 | 1 | 1 | 1.8 | 1Score > 16 indicates identity |
U | R.LSNPELRK.S | |
| 2584 |
643.3278 | 1284.6410 | 1284.6422 | -0.89 | 0 | 27 | 0.006 | 1Score > 17 indicates identity |
U | R.NNLDNAVEQLR.Q | |
| 2679 |
698.3942 | 1394.7738 | 1394.7558 | 13.0 | 0 | 23 | 0.008 | 1Score > 15 indicates identity |
U | R.YNYLINQLNIK.S |
45| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | MMUM_ECOLI | 33 | Homocysteine S-methyltransferase OS=Escherichia coli (strain K12) GN=mmuM PE=1 SV=2 |
46| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RIDA_ECOLI | 33 | Enamine/imine deaminase OS=Escherichia coli (strain K12) GN=ridA PE=1 SV=2 |
47| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HEM3_ECOLI | 32 | Porphobilinogen deaminase OS=Escherichia coli (strain K12) GN=hemC PE=1 SV=2 |
48| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YOAB_ECOLI | 30 | RutC family protein YoaB OS=Escherichia coli (strain K12) GN=yoaB PE=3 SV=1 |
49| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARAA_ECOLI | 28 | L-arabinose isomerase OS=Escherichia coli (strain K12) GN=araA PE=1 SV=3 |
50| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DCUS_ECOLI | 27 | Sensor histidine kinase DcuS OS=Escherichia coli (strain K12) GN=dcuS PE=1 SV=1 |
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