MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:35:06 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLYA_ECOLI | 126 | Serine hydroxymethyltransferase OS=Escherichia coli (strain K12) GN=glyA PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 21.1 | GLYA_ECOLI |
126 | 45459 | 16 (8) | 14 (7) | 0.63 | |
| Serine hydroxymethyltransferase OS=Escherichia coli (strain K12) GN=glyA PE=1 SV=1 | |||||||
16 peptide matches (14 non-duplicate, 2 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 1635 |
612.3055 | 611.2982 | 611.2955 | 4.45 | 0 | 4 | 0.42 | 1Score > 13 indicates identity |
U | R.YPVYA.- | |
| 1637 |
307.6861 | 613.3576 | 613.3548 | 4.72 | 0 | 19 | 0.012 | 1Score > 13 indicates identity |
U | K.TLAGPR.G | |
| 1704 |
336.2275 | 670.4404 | 670.4377 | 4.03 | 0 | 10 | 0.1 | 1Score > 13 indicates identity |
U | R.GGLILAK.G | |
| 1834 |
380.2220 | 758.4294 | 758.4286 | 1.07 | 0 | 33 | 0.0012 | 1Score > 21 indicates identity Score > 16 indicates homology |
U | R.ANITVNK.N | |
| 1893 |
407.7448 | 813.4750 | 813.4709 | 5.15 | 0 | 43 | 0.00016 | 1Score > 18 indicates identity |
U | R.VGTPAITR.R | |
| 1966 |
435.7208 | 869.4270 | 869.4243 | 3.18 | 0 | 4 | 0.65 | 1Score > 14 indicates identity |
U | K.NSVPNDPK.S | |
| 2096 |
1 |
482.2682 | 962.5218 | 962.5185 | 3.44 | 0 | 28 | 0.0057 | 1Score > 18 indicates identity |
U | K.SPFVTSGIR.V |
| 2187 |
498.7238 | 995.4330 | 995.4270 | 6.10 | 0 | 33 | 0.0008 | 1Score > 14 indicates identity |
U | K.EAMEPEFK.T + Oxidation (M) | |
| 2253 |
347.5111 | 1039.5115 | 1039.5087 | 2.70 | 1 | 14 | 0.11 | 1Score > 17 indicates identity |
U | K.YAEGYPGKR.Y | |
| 2331 |
1 |
555.2881 | 1108.5616 | 1108.5587 | 2.69 | 0 | 40 | 0.00037 | 1Score > 18 indicates identity |
U | K.AMVEVFLER.G + Oxidation (M) |
| 2610 |
660.8342 | 1319.6538 | 1319.6503 | 2.65 | 0 | 19 | 0.062 | 1Score > 19 indicates identity |
U | R.VMQAQGSQLTNK.Y + Oxidation (M) | |
| 3389 |
689.7059 | 2066.0959 | 2066.0619 | 16.4 | 0 | 13 | 0.17 | 1Score > 18 indicates identity |
U | K.LNSAVFPGGQGGPLMHVIAGK.A + Deamidated (NQ); Oxidation (M) | |
| 3486 |
549.5520 | 2194.1789 | 2194.1569 | 10.0 | 1 | 14 | 0.076 | 1Score > 15 indicates identity |
U | K.KLNSAVFPGGQGGPLMHVIAGK.A + Deamidated (NQ); Oxidation (M) | |
| 3682 |
611.3333 | 2441.3041 | 2441.2802 | 9.78 | 1 | 19 | 0.026 | 1Score > 16 indicates identity |
U | K.VVSGGTDNHLFLVDLVDKNLTGK.E + Deamidated (NQ) |
22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LPP_ECOLI | 110 | Major outer membrane lipoprotein Lpp OS=Escherichia coli (strain K12) GN=lpp PE=1 SV=1 |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CISY_ECOLI | 106 | Citrate synthase OS=Escherichia coli (strain K12) GN=gltA PE=1 SV=1 |
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_ECOLI | 89 | DNA-directed RNA polymerase subunit beta OS=Escherichia coli (strain K12) GN=rpoB PE=1 SV=1 |
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ENO_ECOLI | 87 | Enolase OS=Escherichia coli (strain K12) GN=eno PE=1 SV=2 |
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GMHA_ECOLI | 85 | Phosphoheptose isomerase OS=Escherichia coli (strain K12) GN=gmhA PE=1 SV=1 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU1_ECOLI | 83 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DEOD_ECOLI | 81 | Purine nucleoside phosphorylase DeoD-type OS=Escherichia coli (strain K12) GN=deoD PE=1 SV=2 |
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | METK_ECOLI | 72 | S-adenosylmethionine synthase OS=Escherichia coli (strain K12) GN=metK PE=1 SV=2 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PURA_ECOLI | 71 | Adenylosuccinate synthetase OS=Escherichia coli (strain K12) GN=purA PE=1 SV=2 |
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