MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:35:06 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YCDX_ECOLI | 208 | Probable phosphatase YcdX OS=Escherichia coli (strain K12) GN=ycdX PE=1 SV=1 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_ECOLI | 205 | ATP synthase subunit beta OS=Escherichia coli (strain K12) GN=atpD PE=1 SV=2 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_ECOLI | 203 | Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HEMX_ECOLI | 193 | Putative uroporphyrinogen-III C-methyltransferase OS=Escherichia coli (strain K12) GN=hemX PE=1 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NQOR_ECOLI | 186 | NAD(P)H dehydrogenase (quinone) OS=Escherichia coli (strain K12) GN=wrbA PE=1 SV=2 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_ECOLI | 180 | Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TNAA_ECOLI | 156 | Tryptophanase OS=Escherichia coli (strain K12) GN=tnaA PE=1 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLPQ_ECOLI | 153 | Glycerophosphoryl diester phosphodiesterase OS=Escherichia coli (strain K12) GN=glpQ PE=1 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 18.1 | GLPQ_ECOLI |
153 | 40874 | 12 (8) | 12 (8) | 0.86 | |
| Glycerophosphoryl diester phosphodiesterase OS=Escherichia coli (strain K12) GN=glpQ PE=1 SV=2 | |||||||
12 peptide matches (12 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 1764 |
354.7311 | 707.4476 | 707.4442 | 4.83 | 0 | 16 | 0.025 | 1Score > 13 indicates identity |
U | K.IVIAHR.G | |
| 1910 |
410.7199 | 819.4252 | 819.4239 | 1.64 | 0 | 14 | 0.15 | 1Score > 18 indicates identity |
U | K.VQTYPGR.F | |
| 1928 |
415.7737 | 829.5328 | 829.5273 | 6.71 | 1 | 27 | 0.0019 | 1Score > 13 indicates identity |
U | K.TLEVLKK.Y | |
| 2266 |
523.7999 | 1045.5852 | 1045.5808 | 4.29 | 0 | 15 | 0.12 | 1Score > 18 indicates identity |
U | K.NIGIYPEIK.A | |
| 2303 |
361.8838 | 1082.6296 | 1082.6237 | 5.45 | 0 | 18 | 0.026 | 1Score > 15 indicates identity |
U | K.LVVHPYTVR.S | |
| 2589 |
430.8881 | 1289.6425 | 1289.6364 | 4.69 | 1 | 5 | 1 | 1Score > 20 indicates identity Score > 18 indicates homology |
U | R.VTDVADRFPDR.A | |
| 2632 |
674.8311 | 1347.6476 | 1347.6453 | 1.77 | 0 | 33 | 0.00085 | 1Score > 20 indicates identity Score > 15 indicates homology |
U | K.LTGMVQDAQQNK.L + Oxidation (M) | |
| 2657 |
691.3452 | 1380.6758 | 1380.6562 | 14.3 | 0 | 78 | 6e-008 | 1Score > 18 indicates identity |
U | K.AGVNGLFTDFPDK.A + Deamidated (NQ) | |
| 2658 |
462.5610 | 1384.6612 | 1384.6510 | 7.31 | 1 | 36 | 0.00095 | 1Score > 18 indicates identity |
U | K.FTEGFDIENGKK.V + Deamidated (NQ) | |
| 2752 |
480.9230 | 1439.7472 | 1439.7409 | 4.38 | 0 | 15 | 0.082 | 1Score > 17 indicates identity |
U | R.GASGYLPEHTLPAK.A | |
| 3568 |
576.2905 | 2301.1329 | 2301.0913 | 18.1 | 0 | 25 | 0.013 | 1Score > 19 indicates identity |
U | R.VHTFEEEIEFVQGLNHSTGK.N + Deamidated (NQ) | |
| 4399 |
772.1720 | 3855.8236 | 3855.7607 | 16.3 | 1 | 33 | 0.0021 | 1Score > 18 indicates identity |
U | K.AMAYAQGADYLEQDLVMTKDDNLVVLHDHYLDR.V + 2 Deamidated (NQ); 2 Oxidation (M) |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PAND_ECOLI | 139 | Aspartate 1-decarboxylase OS=Escherichia coli (strain K12) GN=panD PE=1 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADHP_ECOLI | 134 | Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1 |
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