MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:35:06 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YCDX_ECOLI | 208 | Probable phosphatase YcdX OS=Escherichia coli (strain K12) GN=ycdX PE=1 SV=1 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_ECOLI | 205 | ATP synthase subunit beta OS=Escherichia coli (strain K12) GN=atpD PE=1 SV=2 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_ECOLI | 203 | Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HEMX_ECOLI | 193 | Putative uroporphyrinogen-III C-methyltransferase OS=Escherichia coli (strain K12) GN=hemX PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 14.1 | HEMX_ECOLI |
193 | 42937 | 7 (6) | 6 (5) | 0.45 | |
| Putative uroporphyrinogen-III C-methyltransferase OS=Escherichia coli (strain K12) GN=hemX PE=1 SV=1 | |||||||
7 peptide matches (6 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1902 |
409.2260 | 816.4374 | 816.4341 | 4.08 | 0 | 27 | 0.0098 | 1Score > 20 indicates identity |
U | R.QQETLAK.Q | |
| 2181 |
496.2583 | 990.5020 | 990.4982 | 3.90 | 0 | 47 | 6.5e-005 | 1Score > 18 indicates identity |
U | K.TSAVVEETR.E | |
| 2248 |
1 |
519.3287 | 1036.6428 | 1036.6393 | 3.43 | 0 | 67 | 2.2e-007 | 1Score > 13 indicates identity |
U | R.LLVAAQAVPR.H |
| 2695 |
707.3799 | 1412.7452 | 1412.7372 | 5.72 | 0 | 57 | 6.2e-006 | 1Score > 18 indicates identity |
U | K.LNQLSNQVDNLR.L | |
| 2787 |
738.3668 | 1474.7190 | 1474.7151 | 2.66 | 0 | 16 | 0.041 | 1Score > 20 indicates identity Score > 14 indicates homology |
U | R.EAVDTTSQPVATEK.K | |
| 3658 |
803.4353 | 2407.2841 | 2407.2370 | 19.6 | 0 | 0 | 2.3 | 1Score > 17 indicates identity |
U | R.AITDDIASLSAVSQVDYDGIILK.L + Deamidated (NQ) |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NQOR_ECOLI | 186 | NAD(P)H dehydrogenase (quinone) OS=Escherichia coli (strain K12) GN=wrbA PE=1 SV=2 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_ECOLI | 180 | Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TNAA_ECOLI | 156 | Tryptophanase OS=Escherichia coli (strain K12) GN=tnaA PE=1 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLPQ_ECOLI | 153 | Glycerophosphoryl diester phosphodiesterase OS=Escherichia coli (strain K12) GN=glpQ PE=1 SV=2 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PAND_ECOLI | 139 | Aspartate 1-decarboxylase OS=Escherichia coli (strain K12) GN=panD PE=1 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADHP_ECOLI | 134 | Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1 |
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