MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:35:06 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YCDX_ECOLI | 208 | Probable phosphatase YcdX OS=Escherichia coli (strain K12) GN=ycdX PE=1 SV=1 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_ECOLI | 205 | ATP synthase subunit beta OS=Escherichia coli (strain K12) GN=atpD PE=1 SV=2 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_ECOLI | 203 | Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 13.1 | DNAK_ECOLI |
203 | 69130 | 14 (10) | 13 (9) | 0.52 | |
| Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2 | |||||||
14 peptide matches (13 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 1896 |
408.7177 | 815.4208 | 815.4137 | 8.73 | 0 | 25 | 0.014 | 1Score > 19 indicates identity |
U | K.DQGIDLR.N | |
| 2085 |
476.7452 | 951.4758 | 951.4736 | 2.41 | 0 | 3 | 1 | 1Score > 16 indicates identity |
U | R.DVSIMPFK.I + Oxidation (M) | |
| 2093 |
480.7353 | 959.4560 | 959.4494 | 6.88 | 0 | 30 | 0.004 | 1Score > 18 indicates identity |
U | R.NDPLAMQR.L + Oxidation (M) | |
| 2200 |
501.7468 | 1001.4790 | 1001.4778 | 1.28 | 0 | 11 | 0.22 | 1Score > 17 indicates identity |
U | R.VLENAEGDR.T | |
| 2267 |
525.7484 | 1049.4822 | 1049.4778 | 4.25 | 0 | 29 | 0.0033 | 1Score > 16 indicates identity |
U | R.FQDEEVQR.D | |
| 2457 |
393.8780 | 1178.6122 | 1178.6084 | 3.20 | 1 | 30 | 0.0038 | 1Score > 18 indicates identity |
U | K.VAEFFGKEPR.K | |
| 2680 |
1 |
465.9379 | 1394.7919 | 1394.7809 | 7.86 | 1 | 40 | 0.0001 | 1Score > 13 indicates identity |
U | R.LINYLVEEFKK.D |
| 2728 |
476.6036 | 1426.7890 | 1426.7854 | 2.54 | 1 | 47 | 3.7e-005 | 1Score > 16 indicates identity |
U | K.MAPPQISAEVLKK.M + Oxidation (M) | |
| 2796 |
495.9474 | 1484.8204 | 1484.8198 | 0.37 | 1 | 27 | 0.0061 | 1Score > 17 indicates identity |
U | R.AKLESLVEDLVNR.S | |
| 3034 |
830.4580 | 1658.9014 | 1658.8879 | 8.17 | 0 | 69 | 3.8e-007 | 1Score > 17 indicates identity |
U | R.IINEPTAAALAYGLDK.G | |
| 3681 |
814.7706 | 2441.2900 | 2441.2650 | 10.2 | 0 | 12 | 0.17 | 1Score > 17 indicates identity |
U | K.VALQDAGLSVSDIDDVILVGGQTR.M + Deamidated (NQ) | |
| 3945 |
911.4865 | 2731.4377 | 2731.3916 | 16.9 | 1 | 38 | 0.00043 | 1Score > 16 indicates identity |
U | K.AKIELSSAQQTDVNLPYITADATGPK.H + Deamidated (NQ) | |
| 4061 |
957.4739 | 2869.3999 | 2869.3658 | 11.9 | 0 | 16 | 0.13 | 1Score > 20 indicates identity |
U | K.TAEDYLGEPVTEAVITVPAYFNDAQR.Q + Deamidated (NQ) |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HEMX_ECOLI | 193 | Putative uroporphyrinogen-III C-methyltransferase OS=Escherichia coli (strain K12) GN=hemX PE=1 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NQOR_ECOLI | 186 | NAD(P)H dehydrogenase (quinone) OS=Escherichia coli (strain K12) GN=wrbA PE=1 SV=2 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_ECOLI | 180 | Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TNAA_ECOLI | 156 | Tryptophanase OS=Escherichia coli (strain K12) GN=tnaA PE=1 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLPQ_ECOLI | 153 | Glycerophosphoryl diester phosphodiesterase OS=Escherichia coli (strain K12) GN=glpQ PE=1 SV=2 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PAND_ECOLI | 139 | Aspartate 1-decarboxylase OS=Escherichia coli (strain K12) GN=panD PE=1 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADHP_ECOLI | 134 | Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1 |
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