MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:35:06 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YCDX_ECOLI | 208 | Probable phosphatase YcdX OS=Escherichia coli (strain K12) GN=ycdX PE=1 SV=1 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_ECOLI | 205 | ATP synthase subunit beta OS=Escherichia coli (strain K12) GN=atpD PE=1 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 12.1 | ATPB_ECOLI |
205 | 50351 | 12 (10) | 12 (10) | 0.88 | |
| ATP synthase subunit beta OS=Escherichia coli (strain K12) GN=atpD PE=1 SV=2 | |||||||
12 peptide matches (12 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 1999 |
450.7682 | 899.5218 | 899.5189 | 3.32 | 0 | 29 | 0.0034 | 1Score > 17 indicates identity |
U | R.GVQSILQR.Y | |
| 2095 |
481.2775 | 960.5404 | 960.5393 | 1.20 | 0 | 40 | 0.00037 | 1Score > 18 indicates identity |
U | K.VGLFGGAGVGK.T | |
| 2311 |
365.5459 | 1093.6159 | 1093.6131 | 2.49 | 1 | 15 | 0.039 | 1Score > 13 indicates identity |
U | K.YVSLKDTIR.G | |
| 2384 |
569.7869 | 1137.5592 | 1137.5522 | 6.20 | 0 | 11 | 0.29 | 1Score > 18 indicates identity |
U | K.TVNMMELIR.N + 2 Oxidation (M) | |
| 2414 |
575.3145 | 1148.6144 | 1148.6111 | 2.92 | 0 | 37 | 0.00084 | 1Score > 18 indicates identity |
U | R.VALTGLTMAEK.F + Oxidation (M) | |
| 2575 |
427.2211 | 1278.6415 | 1278.6350 | 5.04 | 1 | 8 | 0.73 | 1Score > 19 indicates identity |
U | R.TIAMGSSDGLRR.G + Oxidation (M) | |
| 2767 |
725.8984 | 1449.7822 | 1449.7827 | -0.33 | 0 | 52 | 1.7e-005 | 1Score > 17 indicates identity |
U | R.YTLAGTEVSALLGR.M | |
| 3183 |
614.3127 | 1839.9163 | 1839.9115 | 2.58 | 0 | 32 | 0.0028 | 1Score > 19 indicates identity |
U | R.QLDPLVVGQEHYDTAR.G | |
| 3187 |
462.0093 | 1844.0081 | 1844.0044 | 2.02 | 1 | 29 | 0.0039 | 1Score > 17 indicates identity |
U | R.GLDVKDLEHPIEVPVGK.A | |
| 3243 |
635.9881 | 1904.9425 | 1904.9381 | 2.31 | 0 | 29 | 0.0067 | 1Score > 19 indicates identity |
U | R.NIAIEHSGYSVFAGVGER.T | |
| 3284 |
980.0166 | 1958.0186 | 1957.9826 | 18.4 | 0 | 36 | 0.001 | 1Score > 19 indicates identity |
U | R.FLSQPFFVAEVFTGSPGK.Y + Deamidated (NQ) | |
| 3598 |
780.3764 | 2338.1074 | 2338.0821 | 10.8 | 0 | 65 | 1.3e-006 | 1Score > 19 indicates identity |
U | R.MPSAVGYQPTLAEEMGVLQER.I + Deamidated (NQ); 2 Oxidation (M) |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_ECOLI | 203 | Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HEMX_ECOLI | 193 | Putative uroporphyrinogen-III C-methyltransferase OS=Escherichia coli (strain K12) GN=hemX PE=1 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NQOR_ECOLI | 186 | NAD(P)H dehydrogenase (quinone) OS=Escherichia coli (strain K12) GN=wrbA PE=1 SV=2 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_ECOLI | 180 | Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TNAA_ECOLI | 156 | Tryptophanase OS=Escherichia coli (strain K12) GN=tnaA PE=1 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLPQ_ECOLI | 153 | Glycerophosphoryl diester phosphodiesterase OS=Escherichia coli (strain K12) GN=glpQ PE=1 SV=2 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PAND_ECOLI | 139 | Aspartate 1-decarboxylase OS=Escherichia coli (strain K12) GN=panD PE=1 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADHP_ECOLI | 134 | Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1 |
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