MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 2 |
| MS data file | : | PRT1258_JBEI_Tube2_A2.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:35:06 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,668 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RHAA_ECOLI | 6577 | L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PEPB_ECOLI | 1618 | Peptidase B OS=Escherichia coli (strain K12) GN=pepB PE=3 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IADA_ECOLI | 651 | Isoaspartyl dipeptidase OS=Escherichia coli (strain K12) GN=iadA PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CODA_ECOLI | 463 | Cytosine deaminase OS=Escherichia coli (strain K12) GN=codA PE=1 SV=3 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ALF1_ECOLI | 333 | Fructose-bisphosphate aldolase class 1 OS=Escherichia coli (strain K12) GN=fbaB PE=1 SV=2 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CATNterm_ECOLX | 324 | Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YGEY_ECOLI | 280 | Uncharacterized protein YgeY OS=Escherichia coli (strain K12) GN=ygeY PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 7.1 | YGEY_ECOLI |
280 | 45288 | 12 (11) | 8 (8) | 0.88 | |
| Uncharacterized protein YgeY OS=Escherichia coli (strain K12) GN=ygeY PE=3 SV=1 | |||||||
12 peptide matches (10 non-duplicate, 2 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1639 |
308.2084 | 614.4022 | 614.4003 | 3.20 | 0 | 19 | 0.015 | 1Score > 13 indicates identity |
U | K.LILEK.A | |
| 1793 |
363.7305 | 725.4464 | 725.4436 | 3.98 | 0 | 19 | 0.012 | 1Score > 13 indicates identity |
U | R.ALPAVQK.A | |
| 2254 |
521.2687 | 1040.5228 | 1040.5179 | 4.80 | 0 | 37 | 0.00038 | 1Score > 15 indicates identity |
U | R.LGYDEFLGK.G | |
| 2579 |
1 |
641.3476 | 1280.6806 | 1280.6765 | 3.26 | 0 | 64 | 7.2e-007 | 1Score > 15 indicates identity |
U | K.ALVNAYEGLFGK.A |
| 2886 |
529.6141 | 1585.8205 | 1585.8134 | 4.48 | 0 | 18 | 0.067 | 1Score > 19 indicates identity |
U | K.MGPILGELQELSQR.L + Oxidation (M) | |
| 2887 |
1 |
793.9232 | 1585.8318 | 1585.8134 | 11.7 | 0 | 73 | 2.1e-007 | 1Score > 19 indicates identity |
U | K.MGPILGELQELSQR.L + Oxidation (M) |
| 2885 |
793.9149 | 1585.8152 | 1585.8134 | 1.18 | 0 | (59) | 6e-006 | 1Score > 19 indicates identity |
U | K.MGPILGELQELSQR.L + Oxidation (M) | |
| 2890 |
794.4165 | 1586.8184 | 1586.7974 | 13.3 | 0 | 19 | 0.018 | 1Score > 20 indicates identity Score > 14 indicates homology |
U | K.MGPILGELQELSQR.L + Deamidated (NQ); Oxidation (M) | |
| 3123 |
594.9887 | 1781.9443 | 1781.9346 | 5.44 | 0 | 28 | 0.0045 | 1Score > 17 indicates identity |
U | R.LVAMDAHIDTVGIGNIK.N + Oxidation (M) | |
| 3545 |
571.0500 | 2280.1709 | 2280.1651 | 2.54 | 1 | 29 | 0.0044 | 1Score > 18 indicates identity |
U | R.HGIPVIGFGPGKEPEAHAPNEK.T | |
| 3701 |
622.5706 | 2486.2533 | 2486.2264 | 10.8 | 1 | 33 | 0.0024 | 1Score > 19 indicates identity |
U | K.VGFDKVEIDPMGNVLGYIGHGPR.L + Deamidated (NQ); Oxidation (M) |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KDSC_ECOLI | 270 | 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC OS=Escherichia coli (strain K12) GN=kdsC PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 263 | Trypsin OS=Sus scrofa PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GLDA_ECOLI | 261 | Glycerol dehydrogenase OS=Escherichia coli (strain K12) GN=gldA PE=1 SV=1 |
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