MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1258_JBEI_Tube2_A2.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:35:06 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,668

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 63)

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+1

Accession Score Description
1 RHAA_ECOLI 6577 L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2

+2

Accession Score Description
1 PEPB_ECOLI 1618 Peptidase B OS=Escherichia coli (strain K12) GN=pepB PE=3 SV=2

-3

Accession Score Description
1 IADA_ECOLI 651 Isoaspartyl dipeptidase OS=Escherichia coli (strain K12) GN=iadA PE=1 SV=1
Score Mass Matches Sequences emPAI
3.1 IADA_ECOLI 651 41400 21 (20) 13 (12) 2.17
Isoaspartyl dipeptidase OS=Escherichia coli (strain K12) GN=iadA PE=1 SV=1

-21 peptide matches (16 non-duplicate, 5 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1871   401.2282 800.4418 800.4392 3.30 0 10 0.41 +1Score > 19 indicates identity U K.DVAIIDR.V
1933   417.7330 833.4514 833.4494 2.40 0 19 0.05 +1Score > 19 indicates identity U R.TITGSVEK.D
1971 +1 436.7478 871.4810 871.4763 5.43 0 56 9.3e-006 +1Score > 18 indicates identity U R.TPEVALSR.L
1982   439.7404 877.4662 877.4657 0.57 0 43 0.00018 +1Score > 18 indicates identity U R.IEQVYAR.G
1990 +1 447.7585 893.5024 893.4970 6.05 0 36 0.00057 +1Score > 16 indicates identity U R.HPESLLAK.T
2078   317.1918 948.5536 948.5505 3.25 0 29 0.0019 +1Score > 14 indicates identity U K.LLPTHVNR.N
2079   475.2845 948.5544 948.5505 4.17 0 17 0.03 +1Score > 14 indicates identity U K.LLPTHVNR.N
2185 +1 498.3043 994.5940 994.5923 1.71 0 35 0.0003 +1Score > 13 indicates identity U R.AVQAGIPLAR.V
2847   511.9437 1532.8093 1532.7987 6.89 0 63 2.1e-006 +1Score > 18 indicates identity U R.NVPLFEQALEFAR.K
2848 -1 767.4147 1532.8148 1532.7987 10.5 0 85 1e-008 +1Score > 18 indicates identity U R.NVPLFEQALEFAR.K
2846   767.4102 1532.8058 1532.7987 4.66 0 (59) 5e-006 +1Score > 19 indicates identity U R.NVPLFEQALEFAR.K
2954   808.9488 1615.8830 1615.8781 3.06 1 63 1.3e-006 +1Score > 17 indicates identity U R.TITGSVEKDVAIIDR.V
2955   539.6378 1615.8916 1615.8781 8.34 1 62 1.5e-006 +1Score > 17 indicates identity U R.TITGSVEKDVAIIDR.V
3156 +1 607.2892 1818.8458 1818.8206 13.8 0 43 0.00016 +1Score > 18 indicates identity U R.SAAPDVYHLANMAAESR.V + Deamidated (NQ); Oxidation (M)
3301   988.0381 1974.0616 1974.0634 -0.86 0 131 2e-013 +1Score > 16 indicates identity U R.LTEAGVTSVVGLLGTDSISR.H
3390   690.3635 2068.0687 2068.0510 8.53 0 30 0.0048 +1Score > 19 indicates identity U K.GEILPGNDADLLVMTPELR.I + Oxidation (M)
3587   773.7225 2318.1457 2318.1001 19.7 0 66 1.6e-006 +1Score > 20 indicates identity U R.ALNEEGISAWMLTGAYHVPSR.T + Deamidated (NQ); Oxidation (M)

+4

Accession Score Description
1 CODA_ECOLI 463 Cytosine deaminase OS=Escherichia coli (strain K12) GN=codA PE=1 SV=3

+5

Accession Score Description
1 ALF1_ECOLI 333 Fructose-bisphosphate aldolase class 1 OS=Escherichia coli (strain K12) GN=fbaB PE=1 SV=2

+6

Accession Score Description
1 CATNterm_ECOLX 324 Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1

+7

Accession Score Description
1 YGEY_ECOLI 280 Uncharacterized protein YgeY OS=Escherichia coli (strain K12) GN=ygeY PE=3 SV=1

+8

Accession Score Description
1 KDSC_ECOLI 270 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC OS=Escherichia coli (strain K12) GN=kdsC PE=1 SV=1

+9

Accession Score Description
1 TRYP_PIG 263 Trypsin OS=Sus scrofa PE=1 SV=1

+10

Accession Score Description
1 GLDA_ECOLI 261 Glycerol dehydrogenase OS=Escherichia coli (strain K12) GN=gldA PE=1 SV=1
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