MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 701–800 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
264 311.1112 310.1039
265 311.1115 310.1042
266 311.1116 310.1043
267 311.1118 310.1045
268 311.1467 310.1394
269 311.1473 310.1400
270 311.1479 310.1406
271 311.1482 310.1409
272 311.1483 310.1410
273 311.1484 310.1411
274 311.1484 310.1411
275 311.1484 310.1411
276 311.1485 310.1412
277 311.1487 310.1414
278 311.1487 310.1414
279 311.1487 310.1414
280 311.1495 310.1422
281 311.1667 310.1594
282 311.1674 310.1601
283 311.1856 310.1783
284 311.1873 310.1800
285 311.2055 310.1982
286 311.2062 310.1989
287 311.2231 310.2158
288 311.2233 310.2160
289 311.2235 310.2162
290 311.2237 310.2164
291 311.2239 310.2166
292 311.2242 310.2169
293 311.2242 310.2169
294 312.0305 311.0232
295 312.0305 311.0232
296 312.0311 311.0238
297 312.0315 311.0242
298 312.0542 311.0469
299 312.0547 311.0474
300 312.1818 311.1745
301 313.0388 312.0315
302 313.0604 312.0531
303 313.0901 312.0828
304 313.0979 312.0906
305 313.1271 312.1198
306 313.1278 312.1205
307 313.1279 312.1206
308 313.1287 312.1214
309 313.1288 312.1215
310 313.1629 312.1556
311 313.1634 312.1561
312 313.1638 312.1565
313 313.1649 312.1576
314 313.1653 312.1580
315 313.1660 312.1587
316 313.1853 312.1780
317 313.2211 312.2138
318 313.2212 312.2139
319 314.0459 313.0386
320 314.0459 313.0386
321 314.0464 313.0391
322 314.0467 313.0394
323 314.0569 313.0496
324 314.0831 313.0758
325 314.0834 313.0761
326 314.1971 313.1898
327 314.1972 313.1899
328 314.1972 313.1899
329 314.1975 313.1902
330 314.1979 313.1906
331 314.1982 313.1909
332 315.0535 314.0462
333 315.0536 314.0463
334 315.0754 314.0681
335 315.1131 314.1058
336 315.1139 314.1066
337 315.1423 314.1350
338 315.1425 314.1352
339 315.1445 314.1372
340 315.1597 314.1524
341 315.1748 314.1675
342 315.1789 314.1716
343 315.1795 314.1722
344 315.1797 314.1724
345 315.1806 314.1733
346 315.2368 314.2295
347 316.0263 315.0190
348 316.2126 315.2053
349 316.2127 315.2054
350 316.2130 315.2057
351 316.2132 315.2059
352 316.2135 315.2062
353 316.2137 315.2064
354 316.2138 315.2065
355 316.2139 315.2066
356 316.2507 315.2434
357 316.2510 315.2437
358 317.0542 316.0469
359 317.0542 316.0469
360 317.1372 316.1299
361 317.1754 316.1681
362 317.1758 316.1685
363 318.1914 317.1841
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