MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
164 307.1531 306.1458
165 307.1531 306.1458
166 307.1532 306.1459
167 307.1538 306.1465
168 307.1540 306.1467
169 307.1541 306.1468
170 307.1541 306.1468
171 307.1542 306.1469
172 307.1543 306.1470
173 307.1545 306.1472
174 307.1545 306.1472
175 307.1901 306.1828
176 307.1901 306.1828
177 307.1901 306.1828
178 307.1905 306.1832
179 307.1907 306.1834
180 307.1907 306.1834
181 307.1909 306.1836
182 307.1909 306.1836
183 307.1909 306.1836
184 307.1912 306.1839
185 307.1912 306.1839
186 307.1913 306.1840
187 307.1913 306.1840
188 307.1914 306.1841
189 307.1915 306.1842
190 307.1916 306.1843
191 307.1916 306.1843
192 307.2102 306.2029
193 308.0354 307.0281
194 308.0361 307.0288
195 308.8895 307.8822
196 309.0659 308.0586
197 309.0943 308.0870
198 309.0944 308.0871
199 309.0948 308.0875
200 309.0951 308.0878
201 309.0953 308.0880
202 309.0954 308.0881
203 309.1310 308.1237
204 309.1312 308.1239
205 309.1316 308.1243
206 309.1319 308.1246
207 309.1321 308.1248
208 309.1323 308.1250
209 309.1324 308.1251
210 309.1324 308.1251
211 309.1324 308.1251
212 309.1328 308.1255
213 309.1329 308.1256
214 309.1330 308.1257
215 309.1330 308.1257
216 309.1330 308.1257
217 309.1684 308.1611
218 309.1687 308.1614
219 309.1688 308.1615
220 309.1688 308.1615
221 309.1690 308.1617
222 309.1690 308.1617
223 309.1691 308.1618
224 309.1693 308.1620
225 309.1696 308.1623
226 309.1696 308.1623
227 309.1698 308.1625
228 309.1698 308.1625
229 309.1698 308.1625
230 309.1700 308.1627
231 309.1700 308.1627
232 309.1701 308.1628
233 309.1703 308.1630
234 309.1704 308.1631
235 309.1878 308.1805
236 309.1889 308.1816
237 309.1889 308.1816
238 309.1893 308.1820
239 309.1893 308.1820
240 309.2057 308.1984
241 309.2064 308.1991
242 309.2064 308.1991
243 309.2067 308.1994
244 309.2072 308.1999
245 309.2073 308.2000
246 309.2074 308.2001
247 309.2074 308.2001
248 309.2078 308.2005
249 309.2079 308.2006
250 309.2079 308.2006
251 309.2079 308.2006
252 309.2080 308.2007
253 310.0149 309.0076
254 310.0512 309.0439
255 310.0512 309.0439
256 311.0824 310.0751
257 311.0825 310.0752
258 311.0901 310.0828
259 311.1104 310.1031
260 311.1107 310.1034
261 311.1108 310.1035
262 311.1109 310.1036
263 311.1112 310.1039
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