MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
64 303.8847 302.8774
65 303.8847 302.8774
66 303.8847 302.8774
67 303.8850 302.8777
68 303.8850 302.8777
69 303.8851 302.8778
70 303.8852 302.8779
71 303.8853 302.8780
72 303.8854 302.8781
73 303.8855 302.8782
74 303.8856 302.8783
75 303.8857 302.8784
76 303.8857 302.8784
77 303.8857 302.8784
78 303.8858 302.8785
79 303.8859 302.8786
80 303.8860 302.8787
81 303.8860 302.8787
82 303.8860 302.8787
83 303.8861 302.8788
84 303.8861 302.8788
85 303.8862 302.8789
86 303.8863 302.8790
87 303.8864 302.8791
88 303.8864 302.8791
89 303.8865 302.8792
90 304.0658 303.0585
91 304.1761 303.1688
92 304.1761 303.1688
93 304.1768 303.1695
94 305.0984 304.0911
95 305.0989 304.0916
96 305.0991 304.0918
97 305.0994 304.0921
98 305.1002 304.0929
99 305.1010 304.0937
100 305.1014 304.0941
101 305.1024 304.0951
102 305.1364 304.1291
103 305.1365 304.1292
104 305.1369 304.1296
105 305.1370 304.1297
106 305.1370 304.1297
107 305.1372 304.1299
108 305.1375 304.1302
109 305.1375 304.1302
110 305.1375 304.1302
111 305.1376 304.1303
112 305.1378 304.1305
113 305.1379 304.1306
114 305.1380 304.1307
115 305.1385 304.1312
116 305.1385 304.1312
117 305.1387 304.1314
118 305.1388 304.1315
119 305.1581 304.1508
120 305.1738 304.1665
121 305.1740 304.1667
122 305.1742 304.1669
123 305.1743 304.1670
124 305.1744 304.1671
125 305.1746 304.1673
126 305.1747 304.1674
127 305.1748 304.1675
128 305.1748 304.1675
129 305.1749 304.1676
130 305.1750 304.1677
131 305.1750 304.1677
132 305.1750 304.1677
133 305.1751 304.1678
134 305.1751 304.1678
135 305.1751 304.1678
136 305.1753 304.1680
137 305.2101 304.2028
138 305.2108 304.2035
139 306.1921 305.1848
140 306.1924 305.1851
141 306.1935 305.1862
142 306.2283 305.2210
143 306.8940 305.8867
144 306.9651 305.9578
145 307.1151 306.1078
146 307.1160 306.1087
147 307.1160 306.1087
148 307.1160 306.1087
149 307.1162 306.1089
150 307.1163 306.1090
151 307.1164 306.1091
152 307.1164 306.1091
153 307.1165 306.1092
154 307.1171 306.1098
155 307.1174 306.1101
156 307.1175 306.1102
157 307.1522 306.1449
158 307.1526 306.1453
159 307.1526 306.1453
160 307.1528 306.1455
161 307.1529 306.1456
162 307.1529 306.1456
163 307.1531 306.1458
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