MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4363 809.2313 4041.1201
4364 674.5275 4041.1213
4365 1011.2888 4041.1261
4366 810.4177 4047.0521
4367 810.4177 4047.0521
4368 810.4190 4047.0586
4369 810.4213 4047.0701
4370 810.4227 4047.0771
4371 1013.0200 4048.0509
4372 1013.0201 4048.0513
4373 1013.0206 4048.0533
4374 1013.0209 4048.0545
4375 1013.0227 4048.0617
4376 1013.0236 4048.0653
4377 1013.0237 4048.0657
4378 1013.0244 4048.0685
4379 1013.0246 4048.0693
4380 1013.0250 4048.0709
4381 1013.0258 4048.0741
4382 1016.7694 4063.0485
4383 1016.7719 4063.0585
4384 1016.7760 4063.0749
4385 1016.7764 4063.0765
4389 690.5372 4137.1795
4393 1037.9779 4147.8825
4394 834.6476 4168.2016
4395 695.7104 4168.2187
4396 695.7107 4168.2205
4397 834.6522 4168.2246
4398 596.4681 4168.2258
4399 834.6525 4168.2261
4400 695.7119 4168.2277
4401 596.4684 4168.2279
4402 695.8751 4169.2069
4403 834.8507 4169.2171
4404 596.6100 4169.2191
4405 695.8774 4169.2207
4407 834.8522 4169.2246
4408 695.8782 4169.2255
4409 695.8786 4169.2279
4410 834.8533 4169.2301
4411 695.8790 4169.2303
4412 695.8793 4169.2321
4413 834.8541 4169.2341
4414 834.9752 4169.8396
4415 596.7547 4170.2320
4416 1044.0532 4172.1837
4417 696.8995 4175.3533
4418 696.9055 4175.3893
4419 697.0692 4176.3715
4420 697.0694 4176.3727
4421 1045.5648 4178.2301
4422 837.0235 4180.0811
4423 837.2185 4181.0561
4424 837.2207 4181.0671
4425 837.2210 4181.0686
4427 837.9691 4184.8091
4428 1053.2720 4209.0589
4429 1055.5024 4217.9805
4430 1062.7363 4246.9161
4431 1072.2860 4285.1149
4432 1079.5626 4314.2213
4433 722.1827 4327.0525
4434 722.3423 4328.0101
4435 722.3477 4328.0425
4436 1084.3219 4333.2585
4437 1086.4985 4341.9649
4438 1090.0226 4356.0613
4439 1090.2528 4356.9821
4440 1091.9969 4363.9585
4441 876.0092 4375.0096
4442 1094.7615 4375.0169
4443 879.2084 4391.0056
4444 1100.7632 4399.0237
4445 1104.0601 4412.2113
4446 886.5918 4427.9226
4448 739.1818 4429.0471
4449 739.1821 4429.0489
4459 892.8277 4459.1021
4460 1120.6066 4478.3973
4461 748.8570 4487.0983
4463 901.4266 4502.0966
4464 751.3605 4502.1193
4465 901.6262 4503.0946
4466 751.5240 4503.1003
4467 751.5240 4503.1003
4468 901.6274 4503.1006
4469 1126.7832 4503.1037
4470 901.6291 4503.1091
4472 751.5262 4503.1135
4473 1126.7863 4503.1161
4475 754.0262 4518.1135
4476 904.8262 4519.0946
4478 904.8278 4519.1026
4479 754.1913 4519.1041
4480 906.0258 4525.0926
4481 755.5201 4527.0769
4482 906.6227 4528.0771
4483 1134.5996 4534.3693
4484 909.2202 4541.0646
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