MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4120 637.7522 3183.7246
4121 1064.8344 3191.4814
4122 639.9350 3194.6386
4123 1066.5455 3196.6147
4124 802.4206 3205.6533
4125 1070.2286 3207.6640
4126 1070.8776 3209.6110
4127 1070.8788 3209.6146
4128 643.3166 3211.5466
4129 803.9081 3211.6033
4130 804.1440 3212.5469
4131 1075.1949 3222.5629
4132 806.6525 3222.5809
4133 808.1378 3228.5221
4134 1077.5483 3229.6231
4135 1079.5487 3235.6243
4136 1079.8855 3236.6347
4137 811.1959 3240.7545
4138 813.7031 3250.7833
4139 1085.8971 3254.6695
4140 1085.9016 3254.6830
4141 1087.5492 3259.6258
4142 1090.1697 3267.4873
4143 1090.9097 3269.7073
4146 548.1218 3282.6871
4147 657.5555 3282.7411
4148 821.6927 3282.7417
4149 657.5574 3282.7506
4151 1100.8401 3299.4985
4152 827.4109 3305.6145
4153 1102.8818 3305.6236
4155 833.4315 3329.6969
4156 833.6791 3330.6873
4157 1111.2415 3330.7027
4158 833.6867 3330.7177
4159 668.3671 3336.7991
4160 835.7000 3338.7709
4161 837.4305 3345.6929
4162 837.4349 3345.7105
4163 839.1767 3352.6777
4164 671.7400 3353.6636
4165 562.2819 3367.6477
4166 674.7373 3368.6501
4170 847.6807 3386.6937
4171 847.9359 3387.7145
4172 1131.5715 3391.6927
4179 1144.5880 3430.7422
4180 1144.5900 3430.7482
4181 860.9514 3439.7765
4182 1147.9241 3440.7505
4183 861.1956 3440.7533
4184 861.1974 3440.7605
4185 689.1613 3440.7701
4186 1148.6195 3442.8367
4187 862.4349 3445.7105
4188 1149.5793 3445.7161
4189 865.1965 3456.7569
4191 1153.2665 3456.7777
4194 692.3668 3456.7976
4195 692.3677 3456.8021
4196 865.4211 3457.6553
4197 865.4522 3457.7797
4198 695.3630 3471.7786
4199 870.4310 3477.6949
4200 696.5482 3477.7046
4203 874.4390 3493.7269
4204 583.4617 3494.7265
4205 699.9536 3494.7316
4206 875.2017 3496.7777
4209 703.1431 3510.6791
4210 703.7710 3513.8186
4211 879.7120 3514.8189
4212 880.1711 3516.6553
4213 707.9534 3534.7306
4214 708.1531 3535.7291
4215 708.1541 3535.7341
4216 708.1541 3535.7341
4217 708.1542 3535.7346
4218 708.1542 3535.7346
4219 708.1544 3535.7356
4220 885.4506 3537.7733
4222 890.2253 3556.8721
4223 891.6398 3562.5301
4224 595.4841 3566.8609
4225 595.4852 3566.8675
4226 595.4859 3566.8717
4227 892.9300 3567.6909
4228 714.5785 3567.8561
4229 595.6530 3567.8743
4230 595.9870 3569.8783
4231 894.7422 3574.9397
4232 895.9639 3579.8265
4233 897.2261 3584.8753
4234 717.9827 3584.8771
4235 598.4875 3584.8813
4236 717.9840 3584.8836
4237 717.9843 3584.8851
4238 598.4882 3584.8855
4239 598.4888 3584.8891
4240 598.4891 3584.8909
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