MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3985 950.8728 2849.5966
3987 951.1717 2850.4933
3988 951.4498 2851.3276
3989 951.8280 2852.4622
3990 953.5359 2857.5859
3991 953.8027 2858.3863
3992 955.1047 2862.2923
3993 716.5804 2862.2925
3994 955.4713 2863.3921
3998 718.3764 2869.4765
3999 718.3793 2869.4881
4000 718.3809 2869.4945
4001 957.7752 2870.3038
4004 959.8305 2876.4697
4005 720.3220 2877.2589
4006 961.1267 2880.3583
4007 961.8138 2882.4196
4008 962.8051 2885.3935
4010 962.8077 2885.4013
4012 962.8680 2885.5822
4013 963.4942 2887.4608
4014 964.7961 2891.3665
4017 965.1849 2892.5329
4018 966.5107 2896.5103
4019 968.5096 2902.5070
4020 969.7795 2906.3167
4021 727.8420 2907.3389
4022 970.1205 2907.3397
4023 728.0968 2908.3581
4025 972.5142 2914.5208
4026 973.5207 2917.5403
4028 975.1093 2922.3061
4031 735.3813 2937.4961
4032 736.3992 2941.5677
4034 982.4757 2944.4053
4035 982.4768 2944.4086
4036 982.4784 2944.4134
4038 982.8143 2945.4211
4039 737.5803 2946.2921
4040 741.5701 2962.2513
4043 990.8626 2969.5660
4044 991.7916 2972.3530
4045 993.5092 2977.5058
4047 996.1182 2985.3328
4048 998.4916 2992.4530
4049 998.4929 2992.4569
4050 998.4938 2992.4596
4055 1005.5077 3013.5013
4056 1006.2286 3015.6640
4057 1006.2334 3015.6784
4058 755.1757 3016.6737
4059 755.3796 3017.4893
4060 1010.1694 3027.4864
4061 1010.1725 3027.4957
4062 1010.2014 3027.5824
4063 757.9063 3027.5961
4064 1010.5536 3028.6390
4065 1010.8846 3029.6320
4066 1010.8891 3029.6455
4067 759.8692 3035.4477
4068 608.0988 3035.4576
4070 762.1566 3044.5973
4072 1017.8791 3050.6155
4073 764.1140 3052.4269
4075 1019.1558 3054.4456
4076 1021.1888 3060.5446
4077 1021.5261 3061.5565
4078 1021.8730 3062.5972
4080 767.6533 3066.5841
4081 767.6623 3066.6201
4082 1023.5391 3067.5955
4083 1024.2290 3069.6652
4084 771.3941 3081.5473
4085 1029.8950 3086.6632
4086 1032.1328 3093.3766
4087 776.1660 3100.6349
4088 776.4235 3101.6649
4090 1038.5499 3112.6279
4091 1042.8687 3125.5843
4092 1044.2053 3129.5941
4093 1047.2241 3138.6505
4095 787.6186 3146.4453
4096 788.1495 3148.5689
4097 788.4006 3149.5733
4098 789.6779 3154.6825
4099 632.1442 3155.6846
4101 632.1459 3155.6931
4102 632.1460 3155.6936
4103 632.1462 3155.6946
4104 789.9312 3155.6957
4105 1053.5018 3157.4836
4106 1055.5129 3163.5169
4107 792.4164 3165.6365
4108 792.4188 3165.6461
4109 792.4217 3165.6577
4110 792.6688 3166.6461
4111 792.6708 3166.6541
4112 792.6713 3166.6561
4118 1061.5188 3181.5346
4119 1061.8590 3182.5552
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