MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3399 1031.0500 2060.0854
3401 692.0297 2073.0673
3402 1037.5422 2073.0698
3403 692.0327 2073.0763
3404 1037.5469 2073.0792
3405 1037.5551 2073.0956
3406 1038.0457 2074.0768
3407 1038.0466 2074.0786
3408 1038.0470 2074.0794
3409 692.3674 2074.0804
3410 692.3679 2074.0819
3411 692.3679 2074.0819
3412 1038.0487 2074.0828
3413 1038.0492 2074.0838
3414 1038.0493 2074.0840
3415 692.3693 2074.0861
3416 1038.0504 2074.0862
3417 692.3711 2074.0915
3418 1038.0552 2074.0958
3419 692.3745 2074.1017
3420 692.3746 2074.1020
3421 692.3828 2074.1266
3422 692.7147 2075.1223
3423 692.7186 2075.1340
3424 694.3432 2080.0078
3425 695.3795 2083.1167
3426 695.6879 2084.0419
3427 522.2750 2085.0709
3430 697.3715 2089.0927
3431 1046.5132 2091.0118
3432 698.7234 2093.1484
3434 700.0028 2096.9866 2096.9473 18.7 1     +0Score > 20 indicates identity
Score > 13 indicates homology		 SNSLSRSTEDQFFMYLR + Deamidated (NQ); Oxidation (M)
3437 1051.0415 2100.0684
3438 526.5460 2102.1549
3439 701.9791 2102.9155
3440 1053.5187 2105.0228 2104.9840 18.4 1     +0Score > 20 indicates identity
Score > 13 indicates homology		 ITQPDKYSGFLNNYSDLK + 3 Deamidated (NQ)
3443 528.9952 2111.9517
3444 705.3548 2113.0426
3445 705.3618 2113.0636
3446 1058.0300 2114.0454
3447 705.7255 2114.1547
3448 1058.0861 2114.1576
3449 1058.5302 2115.0458
3450 1058.5360 2115.0574
3452 529.8006 2115.1733
3453 706.7421 2117.2045
3454 707.7000 2120.0782
3455 1064.5609 2127.1072
3456 710.0441 2127.1105
3457 712.3721 2134.0945
3458 428.0450 2135.1886
3459 428.0452 2135.1896
3460 714.7178 2141.1316
3462 1073.5739 2145.1332
3465 716.6890 2147.0452
3466 717.0559 2148.1459
3468 1080.5603 2159.1060
3469 1081.0662 2160.1178
3470 721.0466 2160.1180
3471 721.0489 2160.1249
3472 721.7164 2162.1274
3473 721.7189 2162.1349
3474 723.0305 2166.0697
3478 1087.5430 2173.0714
3479 725.3680 2173.0822
3481 725.3686 2173.0840
3482 544.5188 2174.0461
3485 546.0294 2180.0885
3486 728.0321 2181.0745
3487 728.0385 2181.0937
3489 546.7772 2183.0797
3490 729.7284 2186.1634
3491 1095.3508 2188.6870
3497 1097.0354 2192.0562
3498 731.6929 2192.0569
3499 549.0215 2192.0569
3500 549.0219 2192.0585
3501 549.0563 2192.1961
3503 732.0757 2193.2053
3504 549.5562 2194.1957
3505 732.4080 2194.2022
3508 735.3515 2203.0327
3509 735.3831 2203.1275
3510 1103.5771 2205.1396
3511 552.7695 2207.0489
3512 552.8040 2207.1869
3513 553.3026 2209.1813
3514 553.3068 2209.1981
3517 739.3424 2215.0054
3519 743.7004 2228.0794
3520 446.8074 2229.0006
3521 558.5085 2230.0049
3522 744.3427 2230.0063
3523 744.3641 2230.0705
3524 744.3895 2230.1467
3525 1116.0844 2230.1542
3528 560.0494 2236.1685
3530 748.6859 2243.0359
3531 561.7676 2243.0413
3533 749.3291 2244.9655
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