MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3248 872.9828 1743.9510
3250 877.4624 1752.9102
3251 585.6271 1753.8595
3252 439.4724 1753.8605
3253 585.9682 1754.8828
3254 585.9741 1754.9005
3256 586.6623 1756.9651
3259 882.4910 1762.9674
3260 589.5788 1765.7146
3261 589.9790 1766.9152
3262 884.4655 1766.9164
3263 884.4765 1766.9384
3264 885.9464 1769.8782
3265 886.4509 1770.8872
3266 886.9500 1771.8854
3267 591.9680 1772.8822
3268 887.4514 1772.8882
3270 892.9344 1783.8542
3271 596.0034 1784.9884
3272 597.3168 1788.9286
3273 897.4597 1792.9048
3274 897.4639 1792.9132
3276 898.4615 1794.9084
3277 600.0292 1797.0658
3279 602.9980 1805.9722
3280 603.3309 1806.9709
3281 603.6440 1807.9102
3283 604.3104 1809.9094
3284 906.4619 1810.9092
3289 912.0096 1822.0046
3290 913.4867 1824.9588
3292 610.3540 1828.0402
3293 915.5264 1829.0382
3295 460.9904 1839.9325
3296 921.0082 1840.0018
3299 464.2429 1852.9425
3301 934.0488 1866.0830
3302 934.0493 1866.0840
3303 934.0521 1866.0896
3304 623.0373 1866.0901
3305 623.3716 1867.0930
3306 624.3322 1869.9748
3307 938.4548 1874.8950
3310 626.3254 1875.9544
3311 627.0197 1878.0373
3312 627.3389 1878.9949
3313 627.6287 1879.8643
3314 470.9763 1879.8761
3315 940.9757 1879.9368
3316 941.0042 1879.9938
3317 627.9666 1880.8780
3318 943.4543 1884.8940
3319 944.9961 1887.9776
3321 947.4972 1892.9798
3323 947.4985 1892.9824
3324 947.5018 1892.9890
3326 947.9966 1893.9786
3328 632.3342 1893.9808
3330 633.0010 1895.9812
3331 633.9980 1898.9722
3334 638.9548 1913.8426
3335 640.6771 1919.0095
3336 641.9739 1922.8999
3337 962.5254 1923.0362
3338 643.3718 1927.0936
3339 643.9432 1928.8078
3340 644.3632 1930.0678
3341 644.6985 1931.0737
3347 978.5371 1955.0596
3351 655.9802 1964.9188
3352 657.6495 1969.9267
3353 658.0220 1971.0442
3354 990.5390 1979.0634
3355 661.3724 1981.0954
3358 662.0060 1982.9962
3362 662.6534 1984.9384
3363 663.3463 1987.0171
3365 663.6834 1988.0284
3367 997.9534 1993.8922
3368 667.0032 1997.9878
3369 1000.0286 1998.0426
3370 667.3609 1999.0609
3371 1002.5417 2003.0688
3372 1002.5426 2003.0706
3373 1003.0139 2004.0132
3374 669.0315 2004.0727
3375 669.0330 2004.0772
3376 669.3363 2004.9871
3377 670.0571 2007.1495
3378 1004.5822 2007.1498
3380 1007.4602 2012.9058
3381 672.0154 2013.0244
3386 1012.5253 2023.0360
3387 675.4053 2023.1941
3388 678.3348 2031.9826
3389 680.0183 2037.0331
3390 682.3597 2044.0573
3391 685.3975 2053.1707
3396 1030.5355 2059.0564
3398 1031.0247 2060.0348
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