MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2616 370.8371 1109.4895
2618 557.3002 1112.5858
2619 371.8694 1112.5864
2625 377.8474 1130.5204
2644 384.5146 1150.5220
2645 385.2168 1152.6286
2646 577.3230 1152.6314
2647 578.8195 1155.6244
2648 578.8210 1155.6274
2650 386.8440 1157.5102
2652 580.2906 1158.5666
2658 585.3145 1168.6144
2664 589.2973 1176.5800
2677 591.8279 1181.6412
2678 395.0754 1182.2044
2680 592.7717 1183.5288
2681 593.3232 1184.6318
2682 396.5320 1186.5742
2683 594.3492 1186.6838
2686 595.3099 1188.6052
2687 597.2966 1192.5786
2690 399.2102 1194.6088
2692 399.2109 1194.6109
2693 598.3839 1194.7532
2694 599.2629 1196.5112
2696 600.2882 1198.5618
2697 601.2766 1200.5386
2700 301.1491 1200.5673
2701 301.1492 1200.5677
2703 301.3911 1201.5353
2704 601.7770 1201.5394
2706 602.7775 1203.5404
2709 603.2985 1204.5824
2715 603.8307 1205.6468
2717 605.3660 1208.7174
2718 605.3668 1208.7190
2719 404.1917 1209.5533
2721 304.6400 1214.5309
2723 405.8510 1214.5312
2725 406.5271 1216.5595
2728 609.8133 1217.6120
2731 612.3555 1222.6964
2734 410.2268 1227.6586
2735 410.2270 1227.6592
2742 410.8394 1229.4964
2743 616.2574 1230.5002
2744 411.5153 1231.5241
2745 617.8101 1233.6056
2746 617.8453 1233.6760
2747 412.8858 1235.6356
2748 412.8866 1235.6380
2750 619.9137 1237.8128
2751 619.9139 1237.8132
2753 415.1986 1242.5740
2754 311.6510 1242.5749
2755 311.6512 1242.5757
2757 415.5172 1243.5298
2758 311.8899 1243.5305
2761 419.1567 1254.4483
2762 419.2076 1254.6010
2764 420.8406 1259.5000
2765 315.9075 1259.6009
2767 315.9079 1259.6025
2768 420.8748 1259.6026
2771 423.2083 1266.6031
2774 636.8087 1271.6028
2789 640.8493 1279.6840
2791 641.8572 1281.6998
2798 430.5503 1288.6291
2807 433.8806 1298.6200
2813 434.8278 1301.4616
2816 653.8101 1305.6056
2817 327.6841 1306.7073
2818 436.5765 1306.7077
2821 436.5775 1306.7107
2823 437.2140 1308.6202
2824 655.3681 1308.7216
2825 438.2015 1311.5827
2826 657.7938 1313.5730
2828 439.1984 1314.5734
2829 329.6515 1314.5769
2830 658.3528 1314.6910
2831 658.3530 1314.6914
2832 658.8226 1315.6306
2833 659.8133 1317.6120
2834 660.3510 1318.6874
2841 664.8884 1327.7622
2842 333.1728 1328.6621
2843 333.6436 1330.5453
2844 444.5229 1330.5469
2848 445.5570 1333.6492
2849 334.4200 1333.6509
2850 445.5583 1333.6531
2851 334.4206 1333.6533
2853 335.4179 1337.6425
2855 670.3112 1338.6078
2856 672.7739 1343.5332
2858 672.7967 1343.5788
2859 673.3473 1344.6800
2860 449.2594 1344.7564
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