MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2398 427.7251 853.4356
2401 859.4166 858.4093
2403 435.1716 868.3286
2404 435.1732 868.3318
2405 435.7520 869.4894
2406 435.7530 869.4914
2407 435.7751 869.5356
2408 435.7762 869.5378
2413 437.7401 873.4656
2416 439.7777 877.5408
2418 881.4757 880.4684
2419 885.5515 884.5442
2427 448.2158 894.4170
2428 449.7417 897.4688
2430 300.5163 898.5271
2431 301.1877 900.5413
2432 301.1880 900.5422
2433 301.1884 900.5434
2439 911.4931 910.4858
2440 304.5291 910.5655
2442 308.5054 922.4944
2446 463.7366 925.4586
2447 309.5325 925.5757
2451 467.7399 933.4652
2452 313.1625 936.4657
2453 313.8290 938.4652
2454 314.0106 939.0100
2457 471.7969 941.5792
2458 471.7988 941.5830
2459 472.2185 942.4224
2464 474.7461 947.4776
2474 957.4947 956.4874
2481 483.2236 964.4326
2483 968.4792 967.4719
2484 484.7433 967.4720
2486 484.7437 967.4728
2487 484.7437 967.4728
2488 484.7453 967.4760
2492 326.1522 975.4348
2493 488.7305 975.4464
2494 488.7306 975.4466
2496 326.8429 977.5069
2497 490.8000 979.5854
2500 982.7652 981.7579
2501 492.7104 983.4062
2502 328.8102 983.4088
2503 492.7432 983.4718
2504 493.2328 984.4510
2505 985.5242 984.5169
2506 493.2659 984.5172
2507 493.2674 984.5202
2512 329.8016 986.3830
2513 494.2391 986.4636
2514 330.8151 989.4235
2515 330.8153 989.4241
2516 495.7196 989.4246
2517 497.7823 993.5500
2519 499.7062 997.3978
2520 333.4733 997.3981
2521 333.4734 997.3984
2526 503.2015 1004.3884
2527 503.2016 1004.3886
2528 503.2957 1004.5768
2529 336.1380 1005.3922
2531 1008.5040 1007.4967
2532 505.2567 1008.4988
2535 506.2578 1010.5010
2536 507.1889 1012.3632
2539 508.7557 1015.4968
2540 509.2435 1016.4724
2541 509.2437 1016.4728
2543 510.7567 1019.4988
2545 341.1836 1020.5290
2551 342.8200 1025.4382
2552 514.7556 1027.4966
2553 343.5160 1027.5262
2561 345.8500 1034.5282
2564 519.3205 1036.6264
2565 520.7211 1039.4276
2566 347.4833 1039.4281
2573 1053.5243 1052.5170
2574 528.2036 1054.3926
2575 352.4715 1054.3927
2576 529.3004 1056.5862
2577 530.2576 1058.5006
2582 533.2998 1064.5850
2590 537.7253 1073.4360
2591 358.8196 1073.4370
2592 358.8212 1073.4418
2595 540.7808 1079.5470
2598 361.8423 1082.5051
2599 363.4830 1087.4272
2600 544.7209 1087.4272
2602 546.5289 1091.0432
2603 365.1201 1092.3385
2606 549.8088 1097.6030
2608 549.8135 1097.6124
2610 552.2034 1102.3922
2611 552.3051 1102.5956
2612 368.8063 1103.3971
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