MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2180 570.7188 569.7115
2181 570.7191 569.7118
2182 571.3573 570.3500
2186 581.2558 580.2485
2187 596.6431 595.6358
2190 301.0870 600.1594
2197 304.1759 606.3372
2198 304.8354 607.6562
2199 304.8357 607.6568
2200 304.8357 607.6568
2201 304.8360 607.6574
2202 304.8360 607.6574
2203 304.8363 607.6580
2204 304.8363 607.6580
2205 304.8363 607.6580
2206 304.8365 607.6584
2207 304.8366 607.6586
2208 304.8367 607.6588
2218 308.8885 615.7624
2224 314.6877 627.3608
2231 324.1744 646.3342
2235 325.3491 648.6836
2236 325.3497 648.6848
2237 325.3498 648.6850
2238 325.3502 648.6858
2239 327.1797 652.3448
2240 327.9355 653.8564
2241 329.6721 657.3296
2242 329.6722 657.3298
2243 330.7006 659.3866
2247 334.7041 667.3936
2249 670.3792 669.3719
2252 676.3060 675.2987
2253 676.4049 675.3976
2262 687.4160 686.4087
2263 346.2234 690.4322
2265 698.0140 697.0067
2266 698.3529 697.3456
2267 698.3544 697.3471
2271 352.0255 702.0364
2272 352.1940 702.3734
2273 355.6877 709.3608
2274 356.2057 710.3968
2278 360.1928 718.3710
2279 721.3836 720.3763
2280 721.3837 720.3764
2281 362.6831 723.3516
2283 363.1795 724.3444
2284 727.3770 726.3697
2291 731.3878 730.3805
2292 366.1981 730.3816
2295 369.1907 736.3668
2296 738.3876 737.3803
2298 369.7121 737.4096
2299 370.6682 739.3218
2300 370.6950 739.3754
2301 371.2169 740.4192
2304 741.4540 740.4467
2305 741.4547 740.4474
2306 372.2199 742.4252
2308 743.4427 742.4354
2309 743.4447 742.4374
2313 373.7095 745.4044
2316 754.3848 753.3775
2317 378.7169 755.4192
2318 378.7169 755.4192
2319 379.2062 756.3978
2320 380.7054 759.3962
2321 383.1868 764.3590
2323 385.1493 768.2840
2338 391.1840 780.3534
2339 391.1848 780.3550
2341 391.6864 781.3582
2342 782.8931 781.8858
2343 392.1650 782.3154
2344 393.7223 785.4300
2346 393.7414 785.4682
2349 788.3795 787.3722
2351 789.3629 788.3556
2354 399.1817 796.3488
2355 399.2004 796.3862
2361 399.7014 797.3882
2365 402.2097 802.4048
2366 803.4149 802.4076
2367 403.2316 804.4486
2369 407.2114 812.4082
2370 407.2124 812.4102
2376 819.4293 818.4220
2377 412.7542 823.4938
2378 414.1792 826.3438
2379 827.4119 826.4046
2381 416.2333 830.4520
2382 418.1887 834.3628
2383 835.9989 834.9916
2386 419.6998 837.3850
2393 423.7110 845.4074
2394 423.7120 845.4094
2395 425.6716 849.3286
2396 850.4293 849.4220
2397 427.6896 853.3646
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