MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2067 469.2440 468.2367
2068 471.1578 470.1505
2069 471.7986 470.7913
2070 471.7996 470.7923
2071 472.1046 471.0973
2072 472.2909 471.2836
2073 472.3255 471.3182
2074 472.7636 471.7563
2075 473.1609 472.1536
2076 474.3300 473.3227
2077 474.3302 473.3229
2078 475.3274 474.3201
2079 479.7145 478.7072
2080 485.8141 484.8068
2081 486.2935 485.2862
2082 486.2946 485.2873
2083 486.7811 485.7738
2084 487.1502 486.1429
2085 488.2733 487.2660
2086 490.2630 489.2557
2087 490.8195 489.8122
2088 490.8208 489.8135
2089 490.8211 489.8138
2090 490.8217 489.8144
2091 490.8218 489.8145
2092 490.8221 489.8148
2093 493.0190 492.0117
2094 493.0200 492.0127
2095 493.0208 492.0135
2096 493.0210 492.0137
2097 493.0211 492.0138
2098 493.0217 492.0144
2099 493.0219 492.0146
2100 493.0220 492.0147
2101 493.0220 492.0147
2102 493.0222 492.0149
2103 493.0226 492.0153
2104 494.8121 493.8048
2105 494.8145 493.8072
2106 494.8147 493.8074
2107 494.8149 493.8076
2108 494.8149 493.8076
2109 494.8152 493.8079
2110 494.8154 493.8081
2111 494.8156 493.8083
2112 494.8156 493.8083
2113 494.8161 493.8088
2114 494.8165 493.8092
2115 494.8165 493.8092
2116 494.8166 493.8093
2117 494.8166 493.8093
2118 494.8174 493.8101
2119 494.8205 493.8132
2120 496.2418 495.2345
2121 496.7146 495.7073
2122 496.7163 495.7090
2123 497.1715 496.1642
2124 498.2742 497.2669
2125 500.1001 499.0928
2128 502.1155 501.1082
2133 516.3162 515.3089
2134 517.1605 516.1532
2136 520.7410 519.7337
2137 521.2509 520.2436
2138 522.0687 521.0614
2139 529.2272 528.2199
2143 534.2598 533.2525
2144 538.2703 537.2630
2145 540.3040 539.2967
2149 547.2642 546.2569
2150 556.2917 555.2844
2151 559.3459 558.3386
2152 559.3462 558.3389
2153 567.2436 566.2363
2154 568.7198 567.7125
2155 568.7206 567.7133
2156 568.7220 567.7147
2157 568.9600 567.9527
2158 570.7141 569.7068
2159 570.7145 569.7072
2160 570.7146 569.7073
2161 570.7149 569.7076
2162 570.7150 569.7077
2163 570.7155 569.7082
2164 570.7155 569.7082
2165 570.7156 569.7083
2166 570.7157 569.7084
2167 570.7159 569.7086
2168 570.7161 569.7088
2169 570.7167 569.7094
2170 570.7169 569.7096
2171 570.7169 569.7096
2172 570.7172 569.7099
2173 570.7173 569.7100
2174 570.7179 569.7106
2175 570.7181 569.7108
2176 570.7183 569.7110
2177 570.7184 569.7111
2178 570.7186 569.7113
2179 570.7186 569.7113
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