MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1864 421.2239 420.2166
1865 421.2240 420.2167
1866 421.2242 420.2169
1867 421.2244 420.2171
1868 423.1344 422.1271
1869 423.1638 422.1565
1870 423.1645 422.1572
1871 423.1993 422.1920
1872 423.1998 422.1925
1873 423.2005 422.1932
1874 423.2008 422.1935
1875 423.2010 422.1937
1876 423.2011 422.1938
1877 423.2014 422.1941
1878 423.2017 422.1944
1879 423.2017 422.1944
1880 423.2022 422.1949
1881 423.2027 422.1954
1882 423.2028 422.1955
1883 423.2029 422.1956
1884 423.2030 422.1957
1885 423.2031 422.1958
1886 423.2198 422.2125
1887 423.2204 422.2131
1888 423.2204 422.2131
1889 423.2208 422.2135
1890 423.2209 422.2136
1891 423.2209 422.2136
1892 423.2210 422.2137
1893 423.2210 422.2137
1894 423.2210 422.2137
1895 423.2211 422.2138
1896 423.2213 422.2140
1897 423.2214 422.2141
1898 423.2215 422.2142
1899 423.2219 422.2146
1900 425.1503 424.1430
1901 425.1801 424.1728
1902 425.1805 424.1732
1903 425.1814 424.1741
1904 425.1817 424.1744
1905 425.1819 424.1746
1906 425.2159 424.2086
1907 425.2165 424.2092
1908 425.2167 424.2094
1909 425.2168 424.2095
1910 425.2185 424.2112
1911 425.2188 424.2115
1912 425.2190 424.2117
1913 425.2351 424.2278
1914 425.2354 424.2281
1915 425.2362 424.2289
1916 425.2362 424.2289
1917 425.2366 424.2293
1918 425.2369 424.2296
1919 425.2369 424.2296
1920 425.2371 424.2298
1921 425.2372 424.2299
1922 425.2373 424.2300
1923 425.2377 424.2304
1924 425.7938 424.7865
1925 426.2077 425.2004
1926 427.1283 426.1210
1927 427.1662 426.1589
1928 428.2522 427.2449
1929 429.1088 428.1015
1930 429.1454 428.1381
1931 429.1457 428.1384
1932 429.1805 428.1732
1934 429.2675 428.2602
1935 429.7379 428.7306
1936 430.0940 429.0867
1937 430.0941 429.0868
1938 430.0952 429.0879
1939 430.2302 429.2229
1940 430.3009 429.2936
1941 430.3012 429.2939
1942 430.3015 429.2942
1943 430.3019 429.2946
1944 430.3020 429.2947
1945 430.3024 429.2951
1946 430.3025 429.2952
1947 430.3026 429.2953
1948 430.3027 429.2954
1949 430.3031 429.2958
1950 433.0654 432.0581
1951 433.0995 432.0922
1952 434.2760 433.2687
1953 434.2772 433.2699
1954 434.3325 433.3252
1955 435.2011 434.1938
1956 435.2022 434.1949
1957 435.2030 434.1957
1958 435.2033 434.1960
1959 435.2033 434.1960
1960 437.1964 436.1891
1961 437.2148 436.2075
1962 437.2163 436.2090
1963 437.2163 436.2090
1964 437.2173 436.2100
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