MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1764 407.2071 406.1998
1765 407.2078 406.2005
1766 407.2082 406.2009
1767 407.2082 406.2009
1768 407.2082 406.2009
1769 407.2083 406.2010
1770 407.2243 406.2170
1771 407.2251 406.2178
1772 407.2252 406.2179
1773 407.2257 406.2184
1774 407.2257 406.2184
1775 407.2258 406.2185
1776 407.2260 406.2187
1777 407.2260 406.2187
1778 407.2261 406.2188
1779 407.2261 406.2188
1780 407.2263 406.2190
1781 407.2264 406.2191
1782 407.2267 406.2194
1783 407.7934 406.7861
1784 407.9919 406.9846
1785 409.1659 408.1586
1786 409.1849 408.1776
1787 409.1851 408.1778
1788 409.1852 408.1779
1789 409.1853 408.1780
1790 409.1854 408.1781
1791 409.1860 408.1787
1792 409.1863 408.1790
1793 409.1866 408.1793
1794 409.1867 408.1794
1795 409.1868 408.1795
1796 409.1868 408.1795
1797 409.1874 408.1801
1798 409.2237 408.2164
1799 409.2238 408.2165
1800 409.2240 408.2167
1801 409.2409 408.2336
1802 409.2417 408.2344
1803 409.2419 408.2346
1804 409.2419 408.2346
1805 409.2419 408.2346
1806 409.5235 408.5162
1807 410.2436 409.2363
1808 410.2438 409.2365
1809 410.2444 409.2371
1810 410.2445 409.2372
1811 410.2445 409.2372
1812 410.2450 409.2377
1813 410.2454 409.2381
1814 410.7905 409.7832
1815 411.1639 410.1566
1816 411.1648 410.1575
1817 411.1652 410.1579
1818 411.1996 410.1923
1819 411.2006 410.1933
1820 411.2007 410.1934
1821 411.2017 410.1944
1822 411.2019 410.1946
1823 411.2025 410.1952
1824 411.2028 410.1955
1825 411.2568 410.2495
1826 412.0235 411.0162
1827 412.2197 411.2124
1828 414.0621 413.0548
1829 414.0621 413.0548
1830 414.0631 413.0558
1831 414.1995 413.1922
1832 415.0915 414.0842
1833 415.1303 414.1230
1834 415.1535 414.1462
1835 415.2140 414.2067
1836 416.0776 415.0703
1837 416.3217 415.3144
1838 416.3763 415.3690
1839 417.0846 416.0773
1840 417.2497 416.2424
1841 418.2472 417.2399
1842 418.2473 417.2400
1843 419.0865 418.0792
1844 419.2082 418.2009
1845 421.0611 420.0538
1846 421.1841 420.1768
1847 421.1863 420.1790
1848 421.1867 420.1794
1849 421.1868 420.1795
1850 421.1869 420.1796
1851 421.1871 420.1798
1852 421.1874 420.1801
1853 421.2043 420.1970
1854 421.2051 420.1978
1855 421.2054 420.1981
1856 421.2054 420.1981
1857 421.2056 420.1983
1858 421.2059 420.1986
1859 421.2204 420.2131
1860 421.2219 420.2146
1861 421.2235 420.2162
1862 421.2237 420.2164
1863 421.2238 420.2165
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