MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1664 393.3013 392.2940
1665 393.3013 392.2940
1666 393.3016 392.2943
1667 395.0760 394.0687
1668 395.1678 394.1605
1669 395.1686 394.1613
1670 395.1686 394.1613
1671 395.1692 394.1619
1672 395.1694 394.1621
1673 395.1696 394.1623
1674 395.1697 394.1624
1675 395.1699 394.1626
1676 395.1700 394.1627
1677 395.1702 394.1629
1678 395.1703 394.1630
1679 395.1711 394.1638
1680 395.1711 394.1638
1681 395.1719 394.1646
1682 395.2045 394.1972
1683 395.2046 394.1973
1684 395.2055 394.1982
1685 395.2061 394.1988
1686 395.2063 394.1990
1687 395.2069 394.1996
1688 395.2072 394.1999
1689 395.2073 394.2000
1690 395.2074 394.2001
1691 395.2081 394.2008
1692 395.2618 394.2545
1693 395.2625 394.2552
1694 395.2628 394.2555
1695 397.0597 396.0524
1696 397.1184 396.1111
1697 397.1502 396.1429
1698 397.1844 396.1771
1699 397.1846 396.1773
1700 397.1846 396.1773
1701 397.1847 396.1774
1702 397.1854 396.1781
1703 397.1856 396.1783
1704 397.1858 396.1785
1705 397.1858 396.1785
1706 397.1858 396.1785
1707 397.1859 396.1786
1708 397.1861 396.1788
1709 397.1866 396.1793
1710 397.1869 396.1796
1711 397.1876 396.1803
1712 397.2214 396.2141
1713 397.2223 396.2150
1714 398.0666 397.0593
1715 398.0670 397.0597
1716 398.0675 397.0602
1717 398.0681 397.0608
1718 398.1391 397.1318
1719 398.2409 397.2336
1720 399.0985 398.0912
1721 400.0467 399.0394
1722 400.0479 399.0406
1723 400.0818 399.0745
1724 400.0838 399.0765
1725 401.1127 400.1054
1726 401.1137 400.1064
1727 401.2875 400.2802
1728 402.2472 401.2399
1729 402.2475 401.2402
1730 402.2476 401.2403
1731 402.2502 401.2429
1732 402.2505 401.2432
1733 402.2520 401.2447
1734 402.2522 401.2449
1735 402.2523 401.2450
1736 402.2838 401.2765
1737 403.0898 402.0825
1738 403.1053 402.0980
1739 403.2315 402.2242
1740 403.2316 402.2243
1741 403.8128 402.8055
1742 404.2269 403.2196
1743 404.2289 403.2216
1744 404.2662 403.2589
1745 405.0703 404.0630
1746 405.1048 404.0975
1747 405.1906 404.1833
1748 405.1919 404.1846
1749 405.1924 404.1851
1750 405.1926 404.1853
1751 405.2278 404.2205
1752 405.2282 404.2209
1753 405.2287 404.2214
1754 405.2295 404.2222
1755 407.1695 406.1622
1756 407.1700 406.1627
1757 407.1700 406.1627
1758 407.1709 406.1636
1759 407.1710 406.1637
1760 407.1711 406.1638
1761 407.2061 406.1988
1762 407.2063 406.1990
1763 407.2065 406.1992
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