MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1564 385.2433 384.2360
1565 385.2444 384.2371
1566 385.9651 384.9578
1567 386.0669 385.0596
1568 386.0676 385.0603
1569 386.0689 385.0616
1570 386.2554 385.2481
1571 386.2563 385.2490
1572 386.2572 385.2499
1573 386.2577 385.2504
1574 387.0746 386.0673
1575 387.0975 386.0902
1576 387.1358 386.1285
1577 387.2005 386.1932
1578 388.2320 387.2247
1579 388.2323 387.2250
1580 388.2342 387.2269
1581 388.2345 387.2272
1582 388.2345 387.2272
1583 388.2361 387.2288
1584 388.2697 387.2624
1585 388.2722 387.2649
1586 388.2725 387.2652
1587 388.2728 387.2655
1588 388.2728 387.2655
1589 388.2729 387.2656
1590 388.2731 387.2658
1591 388.2731 387.2658
1592 388.9635 387.9562
1593 389.0755 388.0682
1594 389.2414 388.2341
1595 389.2526 388.2453
1596 390.2110 389.2037
1597 390.2498 389.2425
1598 390.2507 389.2434
1599 391.1740 390.1667
1600 391.1743 390.1670
1601 391.1762 390.1689
1602 391.1766 390.1693
1603 391.1768 390.1695
1604 391.1768 390.1695
1605 391.2125 390.2052
1606 391.2127 390.2054
1607 391.2300 390.2227
1608 391.2345 390.2272
1609 392.0054 390.9981
1610 393.0890 392.0817
1611 393.1530 392.1457
1612 393.1533 392.1460
1613 393.1535 392.1462
1614 393.1538 392.1465
1615 393.1541 392.1468
1616 393.1547 392.1474
1617 393.1548 392.1475
1618 393.1893 392.1820
1619 393.1900 392.1827
1620 393.1901 392.1828
1621 393.1906 392.1833
1622 393.1907 392.1834
1623 393.1915 392.1842
1624 393.1916 392.1843
1625 393.1918 392.1845
1626 393.1920 392.1847
1627 393.1922 392.1849
1628 393.1925 392.1852
1629 393.1926 392.1853
1630 393.1927 392.1854
1631 393.2124 392.2051
1632 393.2453 392.2380
1633 393.2460 392.2387
1634 393.2470 392.2397
1635 393.2478 392.2405
1636 393.2982 392.2909
1637 393.2983 392.2910
1638 393.2983 392.2910
1639 393.2985 392.2912
1640 393.2987 392.2914
1641 393.2988 392.2915
1642 393.2989 392.2916
1643 393.2990 392.2917
1644 393.2991 392.2918
1645 393.2992 392.2919
1646 393.2993 392.2920
1647 393.2993 392.2920
1648 393.2995 392.2922
1649 393.2996 392.2923
1650 393.2996 392.2923
1651 393.3000 392.2927
1652 393.3001 392.2928
1653 393.3002 392.2929
1654 393.3002 392.2929
1655 393.3005 392.2932
1656 393.3007 392.2934
1657 393.3007 392.2934
1658 393.3011 392.2938
1659 393.3012 392.2939
1660 393.3012 392.2939
1661 393.3013 392.2940
1662 393.3013 392.2940
1663 393.3013 392.2940
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