MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1464 377.1973 376.1900
1465 377.1974 376.1901
1466 378.2071 377.1998
1467 378.2071 377.1998
1468 379.1373 378.1300
1469 379.1379 378.1306
1470 379.1381 378.1308
1471 379.1389 378.1316
1472 379.1396 378.1323
1473 379.1735 378.1662
1474 379.1742 378.1669
1475 379.1743 378.1670
1476 379.1745 378.1672
1477 379.1745 378.1672
1478 379.1747 378.1674
1479 379.1747 378.1674
1480 379.1749 378.1676
1481 379.1755 378.1682
1482 379.1756 378.1683
1483 379.1756 378.1683
1484 379.1757 378.1684
1485 379.1757 378.1684
1486 379.1766 378.1693
1487 379.1766 378.1693
1488 379.1767 378.1694
1489 379.1767 378.1694
1490 379.2115 378.2042
1491 379.2119 378.2046
1492 379.2119 378.2046
1493 379.2126 378.2053
1494 381.1529 380.1456
1495 381.1535 380.1462
1496 381.1536 380.1463
1497 381.1538 380.1465
1498 381.1540 380.1467
1499 381.1540 380.1467
1500 381.1544 380.1471
1501 381.1549 380.1476
1502 381.1553 380.1480
1503 381.1559 380.1486
1504 381.1893 380.1820
1505 381.1895 380.1822
1506 381.1895 380.1822
1507 381.1897 380.1824
1508 381.1899 380.1826
1509 381.1899 380.1826
1510 381.1902 380.1829
1511 381.1902 380.1829
1512 381.1904 380.1831
1513 381.1906 380.1833
1514 381.1906 380.1833
1515 381.1916 380.1843
1516 381.1916 380.1843
1517 381.1917 380.1844
1518 381.1920 380.1847
1519 381.1922 380.1849
1520 381.1923 380.1850
1521 381.1923 380.1850
1522 381.1925 380.1852
1523 381.2094 380.2021
1524 381.2106 380.2033
1525 381.2109 380.2036
1526 381.2111 380.2038
1527 381.2114 380.2041
1528 381.2119 380.2046
1529 381.2121 380.2048
1530 382.1881 381.1808
1531 383.1030 382.0957
1532 383.1039 382.0966
1533 383.1330 382.1257
1534 383.1337 382.1264
1535 383.1689 382.1616
1536 383.1690 382.1617
1537 383.1691 382.1618
1538 383.1694 382.1621
1539 383.1700 382.1627
1540 383.1702 382.1629
1541 383.1703 382.1630
1542 383.1704 382.1631
1543 383.1709 382.1636
1544 383.2049 382.1976
1545 383.2062 382.1989
1546 383.2069 382.1996
1547 383.2070 382.1997
1548 383.2078 382.2005
1549 383.2079 382.2006
1550 383.2080 382.2007
1551 383.2080 382.2007
1552 383.2081 382.2008
1553 383.2262 382.2189
1554 383.2272 382.2199
1555 383.2281 382.2208
1556 383.2281 382.2208
1557 384.0516 383.0443
1558 384.0878 383.0805
1559 385.0826 384.0753
1560 385.1187 384.1114
1561 385.1195 384.1122
1562 385.1868 384.1795
1563 385.2213 384.2140
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