| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 1 |
| MS data file | : | PRT1258_JBEI_Tube1_A1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:33:55 GMT |
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the select summary.
| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,568 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 738.3876 | 737.3803 | 737.3820 | -2.28 | 1 | 6 | 0.48 | 1Score > 15 indicates identity |
YRASNK | |
| 647.3447 | 646.3374 | 646.3286 | 13.7 | 1 | 6 | 1.2 | 1Score > 19 indicates identity |
KENEK | |
| 348.6831 | 695.3516 | 695.3425 | 13.2 | 0 | 6 | 0.55 | 1Score > 16 indicates identity |
MNFIR + Oxidation (M) | |
| 362.7097 | 723.4048 | 723.3989 | 8.19 | 0 | 6 | 0.28 | 1Score > 13 indicates identity |
MIFGLK + Oxidation (M) | |
| 371.2301 | 740.4456 | 740.4432 | 3.28 | 0 | 6 | 0.26 | 1Score > 13 indicates identity |
VANLVPK + Deamidated (NQ) | |
| 372.2206 | 742.4266 | 742.4126 | 18.9 | 0 | 6 | 0.69 | 1Score > 17 indicates identity |
FAHAVAK | |
| 712.3608 | 1422.7070 | 1422.7103 | -2.27 | 0 | 6 | 1 | 1Score > 20 indicates identityScore > 18 indicates homology |
ELGIQADVANVHR + 2 Deamidated (NQ) | |
| 401.1871 | 1200.5395 | 1200.5445 | -4.17 | 0 | 6 | 0.41 | 1Score > 14 indicates identity |
DPNVQMVPER + Deamidated (NQ); Oxidation (M) | |
| 790.9184 | 1579.8222 | 1579.7916 | 19.4 | 1 | 6 | 0.97 | 1Score > 18 indicates identity |
DTPILTFMNKLDR + Deamidated (NQ); Oxidation (M) | |
| 581.6433 | 1741.9081 | 1741.8821 | 14.9 | 0 | 6 | 1.3 | 1Score > 19 indicates identity |
LNLEGLEHIAAFMQR + Deamidated (NQ) | |
| 602.8321 | 1203.6496 | 1203.6281 | 17.9 | 0 | 5 | 0.83 | 1Score > 17 indicates identity |
ELMQGSLASLR | |
| 925.8365 | 2774.4877 | 2774.5192 | -11.3 | 1 | 5 | 0.64 | 1Score > 16 indicates identity |
DDLRHATLVALPVPGHGAGEPIGILTR | |
| 307.1487 | 1224.5657 | 1224.5478 | 14.6 | 1 | 5 | 0.71 | 1Score > 16 indicates identity |
MKNVGDLMQR + 2 Deamidated (NQ); 2 Oxidation (M) | |
| 457.7395 | 913.4644 | 913.4505 | 15.3 | 0 | 5 | 0.61 | 1Score > 16 indicates identity |
NVPQQVAR + 3 Deamidated (NQ) | |
| 767.3773 | 1532.7400 | 1532.7219 | 11.8 | 1 | 5 | 1.3 | 1Score > 19 indicates identity |
RDPNQTEFAQAVR + 2 Deamidated (NQ) | |
| 739.1823 | 4429.0501 | 4429.0174 | 7.39 | 1 | 5 | 0.93 | 1Score > 18 indicates identity |
AEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFK + 2 Deamidated (NQ); Oxidation (M) | |
| 836.4313 | 1670.8480 | 1670.8700 | -13.1 | 1 | 5 | 1.1 | 1Score > 18 indicates identity |
QTGGVVDLSNLERQR | |
| 856.4304 | 2566.2694 | 2566.2461 | 9.08 | 0 | 5 | 1 | 1Score > 20 indicates identityScore > 18 indicates homology |
IAIECHSCIQWLTPALENFHK | |
| 822.7731 | 2465.2975 | 2465.2737 | 9.64 | 1 | 5 | 0.88 | 1Score > 17 indicates identity |
GLDAILVPGGFGYRGVEGMITTAR + Oxidation (M) | |
| 557.2987 | 1112.5828 | 1112.5713 | 10.4 | 0 | 5 | 0.66 | 1Score > 16 indicates identity |
TNNPLIPQSK + 2 Deamidated (NQ) | |
| 383.1871 | 764.3596 | 764.3561 | 4.67 | 0 | 5 | 1 | 1Score > 18 indicates identity |
MGIPMGK + 2 Oxidation (M) | |
| 573.9741 | 1718.9005 | 1718.9203 | -11.5 | 1 | 5 | 1 | 1Score > 20 indicates identityScore > 18 indicates homology |
VNKTLEYLTISPNAR + Deamidated (NQ) | |
| 739.1774 | 4429.0207 | 4429.0174 | 0.75 | 1 | 5 | 0.85 | 1Score > 17 indicates identity |
AEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFK + 2 Deamidated (NQ); Oxidation (M) | |
| 843.1699 | 3368.6505 | 3368.6567 | -1.84 | 0 | 5 | 1.3 | 1Score > 19 indicates identity |
AMQQLLDLPQPPTAVFCHSDVMALGALSQAK + 2 Oxidation (M) | |
| 475.4744 | 2372.3356 | 2372.3355 | 0.033 | 1 | 5 | 0.31 | 1Score > 13 indicates identity |
ILSKLGVTTSWFDPLIGADIVK | |
| 916.4769 | 3661.8785 | 3661.8596 | 5.16 | 1 | 5 | 0.93 | 1Score > 17 indicates identity |
FIQIHLEMEDSLPLVQAHMVADQVEQAILRR + Deamidated (NQ); 2 Oxidation (M) | |
| 485.2403 | 1936.9321 | 1936.9088 | 12.0 | 1 | 5 | 1.1 | 1Score > 18 indicates identity |
NLKENQPLFDMTINDK + 2 Deamidated (NQ); Oxidation (M) | |
| 878.4324 | 3509.7005 | 3509.6521 | 13.8 | 1 | 5 | 1.5 | 1Score > 19 indicates identity |
QNDNAPCPPGWQLSLPAARAFILGDEAQNISR + 4 Deamidated (NQ) | |
| 554.7975 | 1107.5804 | 1107.5746 | 5.25 | 0 | 5 | 1.4 | 1Score > 19 indicates identity |
VNLYALMQR + Deamidated (NQ) | |
| 604.2891 | 1809.8455 | 1809.8423 | 1.75 | 0 | 5 | 1.2 | 1Score > 18 indicates identity |
AMLTLMLQAMGQADAGR + Deamidated (NQ); 2 Oxidation (M) | |
| 1000.9050 | 4999.4886 | 4999.4324 | 11.2 | 1 | 5 | 0.66 | 1Score > 16 indicates identity |
GEIFHFNPGSVSIPKGGNPASYGMLDNDVLSVIALNDQSIIAQVAINP + 5 Deamidated (NQ); Oxidation (M) | |
| 771.7134 | 2312.1184 | 2312.0954 | 9.92 | 1 | 5 | 1.7 | 1Score > 19 indicates identity |
DSIGGIVSSRCTNEEGYLVQK + Deamidated (NQ) | |
| 395.7198 | 789.4250 | 789.4093 | 20.0 | 1 | 5 | 1.2 | 1Score > 18 indicates identity |
SRSNGLR + Deamidated (NQ) | |
| 1149.5927 | 1148.5854 | 1148.5859 | -0.45 | 1 | 5 | 1 | 1Score > 20 indicates identityScore > 17 indicates homology |
AETIRLMGDK + Oxidation (M) | |
| 579.7988 | 1157.5830 | 1157.5677 | 13.3 | 0 | 5 | 1.3 | 1Score > 18 indicates identity |
SSDNVPDVGIR | |
| 924.1385 | 2769.3937 | 2769.4218 | -10.2 | 0 | 5 | 1.7 | 1Score > 19 indicates identity |
IMDLQSAQQLGEIAAAVGLAQNLGALR + 3 Deamidated (NQ); Oxidation (M) | |
| 352.1888 | 1404.7261 | 1404.7282 | -1.52 | 0 | 5 | 1.6 | 1Score > 19 indicates identity |
LSMAQSALSQIVR + 2 Deamidated (NQ) | |
| 752.3885 | 2254.1437 | 2254.1126 | 13.8 | 0 | 5 | 1.6 | 1Score > 19 indicates identity |
IIGGYDFILCSNVLSAVPCR + Deamidated (NQ) | |
| 824.1037 | 2469.2893 | 2469.2434 | 18.6 | 1 | 5 | 1.1 | 1Score > 17 indicates identity |
HPQYNQRTIQNDIMLLQLSR + 2 Deamidated (NQ) | |
| 947.4924 | 1892.9702 | 1892.9414 | 15.2 | 1 | 4 | 1.8 | 1Score > 19 indicates identity |
AYNLTSVRDPNEMLVR + Oxidation (M) | |
| 370.9328 | 1479.7021 | 1479.6841 | 12.1 | 1 | 4 | 1.2 | 1Score > 18 indicates identity |
NEDVKQAFDSAVR + 2 Deamidated (NQ) | |
| 774.4737 | 773.4664 | 773.4647 | 2.26 | 1 | 4 | 0.63 | 1Score > 15 indicates identity |
KLLDSAK | |
| 645.3592 | 644.3519 | 644.3493 | 4.04 | 0 | 4 | 0.69 | 1Score > 21 indicates identityScore > 15 indicates homology |
EQLAGK | |
| 549.8087 | 1097.6028 | 1097.6193 | -15.0 | 0 | 4 | 0.74 | 1Score > 16 indicates identity |
IVSRPEELR | |
| 677.5297 | 3382.6121 | 3382.6207 | -2.54 | 1 | 4 | 1.6 | 1Score > 19 indicates identity |
MPNVDVVGEMVNTMSASRSYQANVEVLNTVK + Deamidated (NQ) | |
| 551.5893 | 1651.7461 | 1651.7492 | -1.87 | 0 | 4 | 1.1 | 1Score > 17 indicates identity |
STEFHPVHYDAHGR | |
| 301.6864 | 601.3582 | 601.3548 | 5.81 | 0 | 4 | 0.77 | 1Score > 21 indicates identityScore > 16 indicates homology |
IVAGSR | |
| 432.2448 | 1293.7126 | 1293.7193 | -5.22 | 0 | 4 | 0.64 | 1Score > 15 indicates identity |
SHNIALIWNVK | |
| 501.2828 | 1500.8266 | 1500.8049 | 14.5 | 1 | 4 | 1.3 | 1Score > 18 indicates identity |
LSNITKVFHQGTR + Deamidated (NQ) | |
| 528.6055 | 1582.7947 | 1582.7951 | -0.26 | 0 | 4 | 1.2 | 1Score > 18 indicates identity |
NIDLGQVTTTHINR + 2 Deamidated (NQ) | |
| 688.6740 | 2063.0002 | 2063.0357 | -17.2 | 0 | 4 | 1 | 1Score > 20 indicates identityScore > 17 indicates homology |
SPMGALVYGSGGLLIDNGGLR + Deamidated (NQ); Oxidation (M) | |
| 567.3001 | 1132.5856 | 1132.5910 | -4.76 | 0 | 4 | 1.3 | 1Score > 18 indicates identity |
LLMNDGLVSR + Oxidation (M) | |
| 746.5969 | 2982.3585 | 2982.3903 | -10.7 | 1 | 4 | 1.3 | 1Score > 18 indicates identity |
QDEQKMVTQVISTVNDPYSDQVVIDK + 4 Deamidated (NQ) | |
| 501.3178 | 500.3105 | 500.3071 | 6.88 | 0 | 4 | 1.8 | 1Score > 19 indicates identity |
VIGGR | |
| 642.3867 | 641.3794 | 641.3860 | -10.3 | 1 | 4 | 0.7 | 1Score > 15 indicates identity |
AGAKAPK | |
| 791.3749 | 1580.7352 | 1580.7538 | -11.7 | 1 | 4 | 1.1 | 1Score > 17 indicates identity |
TAALVMNIDDMSKR + Deamidated (NQ); Oxidation (M) | |
| 687.3524 | 2059.0354 | 2059.0408 | -2.63 | 0 | 4 | 0.72 | 1Score > 20 indicates identityScore > 15 indicates homology |
LEAMLPGYTAALATQPAEGR | |
| 687.3546 | 2059.0420 | 2059.0408 | 0.58 | 0 | 4 | 0.52 | 1Score > 20 indicates identityScore > 14 indicates homology |
LEAMLPGYTAALATQPAEGR | |
| 735.0590 | 2202.1552 | 2202.1367 | 8.37 | 1 | 4 | 1.6 | 1Score > 19 indicates identity |
KPWLQALNDAAFAMQRTNK | |
| 617.9987 | 3701.9485 | 3701.8875 | 16.5 | 1 | 4 | 0.59 | 1Score > 14 indicates identity |
LKPQNNLPELISGWRGELMAEALHNLLQEYPQ + Deamidated (NQ) | |
| 910.1261 | 2727.3565 | 2727.3571 | -0.24 | 1 | 4 | 1.7 | 1Score > 19 indicates identity |
SNLLGPRDANGIPVPMTVDESIASMK + Oxidation (M) | |
| 486.7931 | 971.5716 | 971.5651 | 6.73 | 1 | 4 | 0.71 | 1Score > 15 indicates identity |
LEAGNVKLK + Deamidated (NQ) | |
| 886.4363 | 5312.5741 | 5312.5281 | 8.67 | 1 | 4 | 1.2 | 1Score > 17 indicates identity |
MVGQTIQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAK + 6 Deamidated (NQ) | |
| 531.3276 | 530.3203 | 530.3176 | 5.09 | 1 | 4 | 1 | 1Score > 23 indicates identityScore > 16 indicates homology |
QAGKK | |
| 731.8333 | 2923.3041 | 2923.3341 | -10.3 | 1 | 4 | 0.79 | 1Score > 15 indicates identity |
AHVGDEEHRAMLEQQAWIGLMDQAR + Deamidated (NQ); 2 Oxidation (M) | |
| 440.7383 | 879.4620 | 879.4702 | -9.22 | 1 | 4 | 1.5 | 1Score > 18 indicates identity |
FLSKQEK + Deamidated (NQ) | |
| 1139.5736 | 3415.6990 | 3415.7003 | -0.40 | 1 | 4 | 1.6 | 1Score > 19 indicates identity |
DDAIRMGIQVGTPVTLYNPPQLLANDLVCSK + 2 Deamidated (NQ); Oxidation (M) | |
| 739.1827 | 4429.0525 | 4429.1393 | -19.6 | 1 | 4 | 1.4 | 1Score > 18 indicates identity |
INIVDTPGHADFGGEVERVMSMVDSVLLVVDAFDGPMPQTR + Oxidation (M) | |
| 345.8503 | 1034.5291 | 1034.5331 | -3.92 | 1 | 4 | 1.7 | 1Score > 19 indicates identity |
QWKGLMTR + Oxidation (M) | |
| 381.1891 | 1140.5455 | 1140.5233 | 19.4 | 0 | 4 | 1.2 | 1Score > 17 indicates identity |
QALMGGFASSR + Deamidated (NQ); Oxidation (M) | |
| 692.3627 | 3456.7771 | 3456.7203 | 16.4 | 0 | 4 | 1.4 | 1Score > 18 indicates identity |
MGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSR + 2 Deamidated (NQ); Oxidation (M) | |
| 452.2126 | 902.4106 | 902.4280 | -19.2 | 1 | 4 | 0.89 | 1Score > 16 indicates identity |
MPKEQDR | |
| 562.2930 | 1683.8572 | 1683.8428 | 8.56 | 0 | 4 | 1.7 | 1Score > 19 indicates identity |
LTGVGAGASPNNLLDQR + 2 Deamidated (NQ) | |
| 768.3782 | 2302.1128 | 2302.1038 | 3.88 | 1 | 4 | 2.1 | 1Score > 20 indicates identity |
SSILDATIFLADKNDFAAMNK + 2 Deamidated (NQ); Oxidation (M) | |
| 904.4343 | 3613.7081 | 3613.7129 | -1.34 | 1 | 4 | 1.6 | 1Score > 18 indicates identity |
ATVPVITHWDHGRSMEIIHNAWTHGMNSLMR + Deamidated (NQ); Oxidation (M) | |
| 587.2916 | 1172.5686 | 1172.5673 | 1.14 | 0 | 4 | 1.1 | 1Score > 16 indicates identity |
VLNNGDLGENK + Deamidated (NQ) | |
| 623.3338 | 1244.6530 | 1244.6435 | 7.70 | 0 | 4 | 2.2 | 1Score > 19 indicates identity |
VSELPVLMGER + Oxidation (M) | |
| 697.8915 | 2787.5369 | 2787.5615 | -8.84 | 1 | 4 | 0.48 | 1Score > 13 indicates identity |
TRPLLVVLPKQMEQVTAILAVCHR + Oxidation (M) | |
| 739.3475 | 4430.0413 | 4430.1233 | -18.5 | 1 | 4 | 1.3 | 1Score > 17 indicates identity |
INIVDTPGHADFGGEVERVMSMVDSVLLVVDAFDGPMPQTR + Deamidated (NQ); Oxidation (M) | |
| 855.9034 | 1709.7922 | 1709.7773 | 8.75 | 0 | 4 | 1.7 | 1Score > 18 indicates identity |
GWIYQFHYHGMVR + Deamidated (NQ); Oxidation (M) | |
| 373.7107 | 745.4068 | 745.4156 | -11.8 | 0 | 3 | 1 | 1Score > 20 indicates identityScore > 16 indicates homology |
VLEVMR | |
| 990.1326 | 2967.3760 | 2967.4286 | -17.7 | 1 | 3 | 1.8 | 1Score > 19 indicates identity |
GLVAMNMMEGTLVQIRANAVVMATGGAGR + 2 Deamidated (NQ); 3 Oxidation (M) | |
| 443.5288 | 1327.5646 | 1327.5649 | -0.26 | 0 | 3 | 0.57 | 1Score > 13 indicates identity |
CINTEGGYVCR | |
| 444.8856 | 1331.6350 | 1331.6187 | 12.3 | 1 | 3 | 2.1 | 1Score > 19 indicates identity |
QMSHMARNSVR + Oxidation (M) | |
| 875.4128 | 874.4055 | 874.4144 | -10.2 | 1 | 3 | 1.1 | 1Score > 16 indicates identity |
NENRDVK + Deamidated (NQ) | |
| 554.2574 | 1106.5002 | 1106.5138 | -12.3 | 1 | 3 | 1.3 | 1Score > 17 indicates identity |
MNEDASRIR + Oxidation (M) | |
| 493.7412 | 985.4678 | 985.4716 | -3.81 | 0 | 3 | 0.82 | 1Score > 15 indicates identity |
AENGEEPIK | |
| 704.6567 | 2110.9483 | 2110.9331 | 7.20 | 0 | 3 | 1.3 | 1Score > 17 indicates identity |
WSINDFNAITEDQVNSQK + 3 Deamidated (NQ) | |
| 464.7669 | 927.5192 | 927.5137 | 5.93 | 1 | 3 | 0.91 | 1Score > 15 indicates identity |
ETNPRLAK | |
| 912.4638 | 2734.3696 | 2734.3715 | -0.69 | 1 | 3 | 2.2 | 1Score > 19 indicates identity |
GQNFPVLYNQLPDRNAIVFATNDK + Deamidated (NQ) | |
| 890.4293 | 1778.8440 | 1778.8291 | 8.40 | 0 | 3 | 2.1 | 1Score > 19 indicates identity |
SLCQSMIDNQQLVSR + Deamidated (NQ) | |
| 559.8018 | 2235.1781 | 2235.1786 | -0.25 | 1 | 3 | 2 | 1Score > 19 indicates identity |
SFEPLENALAHVKNIIATPAV + 2 Deamidated (NQ) | |
| 721.1830 | 3600.8786 | 3600.8254 | 14.8 | 1 | 3 | 1 | 1Score > 16 indicates identity |
EQAVLMTYYRNNIAHMLVLPSLMAAIVTQHR + 2 Deamidated (NQ); Oxidation (M) | |
| 365.5296 | 1093.5670 | 1093.5767 | -8.93 | 0 | 3 | 1.9 | 1Score > 18 indicates identity |
ILYASETAAR | |
| 643.5682 | 2570.2437 | 2570.2145 | 11.4 | 1 | 3 | 2.5 | 1Score > 20 indicates identity |
NAGLTSAEVSAVIKAMQWQMDFR + 2 Deamidated (NQ); Oxidation (M) | |
| 874.4230 | 3493.6629 | 3493.6163 | 13.3 | 1 | 3 | 2.1 | 1Score > 19 indicates identity |
LIAPGADGVKMQCDLLSCQNAGWQGVTLNTTR + 4 Deamidated (NQ); Oxidation (M) | |
| 750.4429 | 2248.3069 | 2248.2652 | 18.6 | 1 | 3 | 0.51 | 1Score > 13 indicates identity |
LTGNTLEVAQLHSTLGRIIGR | |
| 394.2151 | 786.4156 | 786.4058 | 12.5 | 0 | 3 | 2.4 | 1Score > 19 indicates identity |
MGVHSLK + Oxidation (M) | |
| 581.0013 | 2319.9761 | 2319.9987 | -9.75 | 0 | 3 | 0.52 | 1Score > 13 indicates identity |
MQQQLGDQYSELAANEGYMK + Deamidated (NQ); Oxidation (M) | |
| 698.3453 | 2789.3521 | 2789.3847 | -11.7 | 1 | 3 | 2.2 | 1Score > 19 indicates identity |
GLQLIGWRDMQHALDYLFADGQLK + 2 Deamidated (NQ) |
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the select summary.