MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1364 370.2305 369.2232
1365 370.2615 369.2542
1366 370.2622 369.2549
1367 370.2623 369.2550
1368 370.2628 369.2555
1369 370.2628 369.2555
1370 370.8373 369.8300
1371 370.8385 369.8312
1372 371.1020 370.0947
1373 371.1024 370.0951
1374 371.1031 370.0958
1375 371.1388 370.1315
1376 371.2299 370.2226
1377 371.3157 370.3084
1378 371.3161 370.3088
1379 371.3162 370.3089
1380 371.3167 370.3094
1381 371.3167 370.3094
1382 371.3168 370.3095
1383 371.3168 370.3095
1384 371.3169 370.3096
1385 371.3170 370.3097
1386 371.3170 370.3097
1387 371.3171 370.3098
1388 371.3172 370.3099
1389 371.3172 370.3099
1390 371.3173 370.3100
1391 371.3174 370.3101
1392 371.3175 370.3102
1393 371.3176 370.3103
1394 371.3178 370.3105
1395 371.3179 370.3106
1396 371.3179 370.3106
1397 371.3179 370.3106
1398 371.3180 370.3107
1399 371.3181 370.3108
1400 371.3182 370.3109
1401 371.3185 370.3112
1402 371.3185 370.3112
1403 371.3185 370.3112
1404 371.3186 370.3113
1405 371.3187 370.3114
1406 371.3188 370.3115
1407 371.3189 370.3116
1408 371.3189 370.3116
1409 371.3190 370.3117
1410 371.3190 370.3117
1411 371.3195 370.3122
1412 372.0510 371.0437
1413 372.0511 371.0438
1414 372.0512 371.0439
1415 372.0520 371.0447
1416 372.0520 371.0447
1417 372.0521 371.0448
1418 372.2604 371.2531
1419 372.2777 371.2704
1420 372.2777 371.2704
1421 372.2780 371.2707
1422 372.2784 371.2711
1423 372.2786 371.2713
1424 373.0594 372.0521
1425 373.0799 372.0726
1426 373.0811 372.0738
1427 373.0949 372.0876
1428 373.2206 372.2133
1429 374.0673 373.0600
1430 374.2180 373.2107
1431 374.2189 373.2116
1432 374.2193 373.2120
1433 374.2925 373.2852
1434 374.2927 373.2854
1435 374.2929 373.2856
1436 374.2930 373.2857
1437 374.2931 373.2858
1438 374.2932 373.2859
1439 374.2935 373.2862
1440 374.2936 373.2863
1441 374.2938 373.2865
1442 374.2940 373.2867
1443 375.0600 374.0527
1444 375.0952 374.0879
1445 376.2344 375.2271
1446 376.2350 375.2277
1447 376.2353 375.2280
1448 376.2358 375.2285
1449 377.0556 376.0483
1450 377.1579 376.1506
1451 377.1579 376.1506
1452 377.1585 376.1512
1453 377.1587 376.1514
1454 377.1591 376.1518
1455 377.1593 376.1520
1456 377.1594 376.1521
1457 377.1599 376.1526
1458 377.1602 376.1529
1459 377.1602 376.1529
1460 377.1607 376.1534
1461 377.1610 376.1537
1462 377.1971 376.1898
1463 377.1972 376.1899
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