MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1264 365.2167 364.2094
1265 365.2167 364.2094
1266 365.2169 364.2096
1267 365.2169 364.2096
1268 365.2174 364.2101
1269 366.0415 365.0342
1270 367.1371 366.1298
1271 367.1374 366.1301
1272 367.1375 366.1302
1273 367.1380 366.1307
1274 367.1380 366.1307
1275 367.1382 366.1309
1276 367.1382 366.1309
1277 367.1385 366.1312
1278 367.1386 366.1313
1279 367.1739 366.1666
1280 367.1740 366.1667
1281 367.1741 366.1668
1282 367.1743 366.1670
1283 367.1743 366.1670
1284 367.1744 366.1671
1285 367.1745 366.1672
1286 367.1748 366.1675
1287 367.1748 366.1675
1288 367.1750 366.1677
1289 367.1750 366.1677
1290 367.1750 366.1677
1291 367.1753 366.1680
1292 367.1754 366.1681
1293 367.1761 366.1688
1294 367.1762 366.1689
1295 367.1766 366.1693
1296 367.1766 366.1693
1297 367.1928 366.1855
1298 367.1931 366.1858
1299 367.1935 366.1862
1300 367.1935 366.1862
1301 367.2111 366.2038
1302 367.2115 366.2042
1303 367.2120 366.2047
1304 367.2122 366.2049
1305 367.2129 366.2056
1306 367.2130 366.2057
1307 367.2133 366.2060
1308 367.2314 366.2241
1309 367.2317 366.2244
1310 367.2323 366.2250
1311 367.2325 366.2252
1312 367.2327 366.2254
1313 367.2328 366.2255
1314 367.2329 366.2256
1315 367.2330 366.2257
1316 367.2332 366.2259
1317 367.2333 366.2260
1318 367.2337 366.2264
1319 368.0567 367.0494
1320 369.0872 368.0799
1321 369.0878 368.0805
1322 369.1219 368.1146
1323 369.1526 368.1453
1324 369.1530 368.1457
1325 369.1532 368.1459
1326 369.1534 368.1461
1327 369.1540 368.1467
1328 369.1541 368.1468
1329 369.1543 368.1470
1330 369.1544 368.1471
1331 369.1548 368.1475
1332 369.1556 368.1483
1333 369.1717 368.1644
1334 369.1902 368.1829
1335 369.1902 368.1829
1336 369.1916 368.1843
1337 369.1919 368.1846
1338 369.1920 368.1847
1339 369.1923 368.1850
1340 369.2091 368.2018
1341 369.2265 368.2192
1342 369.2275 368.2202
1343 369.2276 368.2203
1344 369.2279 368.2206
1345 369.2280 368.2207
1346 369.2281 368.2208
1347 369.2283 368.2210
1348 369.2284 368.2211
1349 369.2289 368.2216
1350 369.2476 368.2403
1351 369.2481 368.2408
1352 369.2484 368.2411
1353 369.2487 368.2414
1354 369.2489 368.2416
1355 369.2490 368.2417
1356 369.2491 368.2418
1357 369.2495 368.2422
1358 370.0269 369.0196
1359 370.0282 369.0209
1360 370.0718 369.0645
1361 370.0722 369.0649
1362 370.0727 369.0654
1363 370.0731 369.0658
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