MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1164 360.2029 359.1956
1165 360.2038 359.1965
1166 360.2390 359.2317
1167 360.2396 359.2323
1168 360.2399 359.2326
1169 360.2399 359.2326
1170 360.2404 359.2331
1171 360.3130 359.3057
1172 361.0788 360.0715
1173 361.1635 360.1562
1174 361.1635 360.1562
1175 361.1636 360.1563
1176 361.1644 360.1571
1177 361.1647 360.1574
1178 361.1652 360.1579
1179 361.1653 360.1580
1180 361.1655 360.1582
1181 361.1655 360.1582
1182 361.2019 360.1946
1183 361.2452 360.2379
1184 361.2454 360.2381
1185 361.2455 360.2382
1186 362.2189 361.2116
1187 362.7855 361.7782
1188 363.1053 362.0980
1189 363.1427 362.1354
1190 363.1429 362.1356
1191 363.1432 362.1359
1192 363.1433 362.1360
1193 363.1435 362.1362
1194 363.1437 362.1364
1195 363.1442 362.1369
1196 363.1444 362.1371
1197 363.1791 362.1718
1198 363.1792 362.1719
1199 363.1793 362.1720
1200 363.1797 362.1724
1201 363.1798 362.1725
1202 363.1805 362.1732
1203 363.1806 362.1733
1204 363.1806 362.1733
1205 363.1807 362.1734
1206 363.1809 362.1736
1207 363.1810 362.1737
1208 363.1810 362.1737
1209 363.1810 362.1737
1210 363.1810 362.1737
1211 363.1812 362.1739
1212 363.1813 362.1740
1213 363.1813 362.1740
1214 363.1814 362.1741
1215 363.1815 362.1742
1216 363.1993 362.1920
1217 363.2167 362.2094
1218 364.2335 363.2262
1219 364.2336 363.2263
1220 364.2342 363.2269
1221 364.2342 363.2269
1222 365.1578 364.1505
1223 365.1578 364.1505
1224 365.1582 364.1509
1225 365.1584 364.1511
1226 365.1584 364.1511
1227 365.1586 364.1513
1228 365.1587 364.1514
1229 365.1587 364.1514
1230 365.1587 364.1514
1231 365.1590 364.1517
1232 365.1593 364.1520
1233 365.1598 364.1525
1234 365.1606 364.1533
1235 365.1607 364.1534
1236 365.1608 364.1535
1237 365.1944 364.1871
1238 365.1950 364.1877
1239 365.1951 364.1878
1240 365.1954 364.1881
1241 365.1955 364.1882
1242 365.1959 364.1886
1243 365.1960 364.1887
1244 365.1961 364.1888
1245 365.1962 364.1889
1246 365.1962 364.1889
1247 365.1963 364.1890
1248 365.1963 364.1890
1249 365.1964 364.1891
1250 365.1968 364.1895
1251 365.1968 364.1895
1252 365.1968 364.1895
1253 365.1969 364.1896
1254 365.1970 364.1897
1255 365.1970 364.1897
1256 365.1972 364.1899
1257 365.1972 364.1899
1258 365.1975 364.1902
1259 365.2147 364.2074
1260 365.2157 364.2084
1261 365.2161 364.2088
1262 365.2162 364.2089
1263 365.2164 364.2091
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