MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1064 353.0929 352.0856
1065 353.0934 352.0861
1066 353.1056 352.0983
1067 353.1199 352.1126
1068 353.1216 352.1143
1069 353.1222 352.1149
1070 353.1222 352.1149
1071 353.1223 352.1150
1072 353.1232 352.1159
1073 353.1582 352.1509
1074 353.1585 352.1512
1075 353.1586 352.1513
1076 353.1586 352.1513
1077 353.1587 352.1514
1078 353.1589 352.1516
1079 353.1589 352.1516
1080 353.1589 352.1516
1081 353.1592 352.1519
1082 353.1594 352.1521
1083 353.1595 352.1522
1084 353.1599 352.1526
1085 353.1603 352.1530
1086 353.1947 352.1874
1087 353.1955 352.1882
1088 353.2526 352.2453
1089 353.2530 352.2457
1090 353.2535 352.2462
1091 353.2537 352.2464
1092 353.2538 352.2465
1093 353.2541 352.2468
1094 353.2542 352.2469
1095 353.8354 352.8281
1096 354.0404 353.0331
1097 354.0410 353.0337
1098 354.0414 353.0341
1099 354.0417 353.0344
1100 355.0711 354.0638
1101 355.0713 354.0640
1102 355.1084 354.1011
1103 355.1084 354.1011
1104 355.1089 354.1016
1105 355.1090 354.1017
1106 355.1366 354.1293
1107 355.1369 354.1296
1108 355.1375 354.1302
1109 355.1377 354.1304
1110 355.1381 354.1308
1111 355.1384 354.1311
1112 355.1391 354.1318
1113 355.1740 354.1667
1114 355.1744 354.1671
1115 355.1750 354.1677
1116 355.1751 354.1678
1117 355.1752 354.1679
1118 355.1756 354.1683
1119 355.2100 354.2027
1120 356.0561 355.0488
1121 356.0565 355.0492
1122 356.0568 355.0495
1123 356.0579 355.0506
1124 356.0580 355.0507
1125 356.2809 355.2736
1126 356.2818 355.2745
1127 356.2820 355.2747
1128 356.2820 355.2747
1129 356.2822 355.2749
1130 356.2825 355.2752
1131 356.2826 355.2753
1132 356.2826 355.2753
1133 356.2827 355.2754
1134 356.2828 355.2755
1135 356.2830 355.2757
1136 356.2830 355.2757
1137 356.2835 355.2762
1138 357.0868 356.0795
1139 357.0871 356.0798
1140 357.0872 356.0799
1141 357.1248 356.1175
1142 357.1537 356.1464
1143 357.1550 356.1477
1144 357.2505 356.2432
1145 357.2515 356.2442
1146 358.0357 357.0284
1147 358.0363 357.0290
1148 358.0375 357.0302
1149 358.2234 357.2161
1150 358.2235 357.2162
1151 358.2238 357.2165
1152 358.2243 357.2170
1153 358.2244 357.2171
1154 358.2247 357.2174
1155 358.2252 357.2179
1156 359.0651 358.0578
1157 359.0800 358.0727
1158 359.1115 358.1042
1159 359.1117 358.1044
1160 359.1125 358.1052
1161 359.2054 358.1981
1162 360.2023 359.1950
1163 360.2029 359.1956
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