MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
964 349.1646 348.1573
965 349.1648 348.1575
966 349.1650 348.1577
967 349.1653 348.1580
968 349.1653 348.1580
969 349.1653 348.1580
970 349.1654 348.1581
971 349.1654 348.1581
972 349.1657 348.1584
973 349.1844 348.1771
974 349.1990 348.1917
975 349.1998 348.1925
976 349.1998 348.1925
977 349.2000 348.1927
978 349.2007 348.1934
979 349.2008 348.1935
980 349.2009 348.1936
981 349.2011 348.1938
982 349.2012 348.1939
983 349.2013 348.1940
984 349.2014 348.1941
985 349.2017 348.1944
986 349.2017 348.1944
987 349.2017 348.1944
988 349.2018 348.1945
989 349.2018 348.1945
990 349.2019 348.1946
991 349.2023 348.1950
992 349.2212 348.2139
993 349.2216 348.2143
994 349.2216 348.2143
995 349.2218 348.2145
996 349.2218 348.2145
997 349.2220 348.2147
998 349.2221 348.2148
999 349.2225 348.2152
1000 349.2227 348.2154
1001 349.2229 348.2156
1002 351.1419 350.1346
1003 351.1422 350.1349
1004 351.1422 350.1349
1005 351.1427 350.1354
1006 351.1428 350.1355
1007 351.1430 350.1357
1008 351.1432 350.1359
1009 351.1433 350.1360
1010 351.1437 350.1364
1011 351.1440 350.1367
1012 351.1441 350.1368
1013 351.1442 350.1369
1014 351.1785 350.1712
1015 351.1785 350.1712
1016 351.1790 350.1717
1017 351.1792 350.1719
1018 351.1793 350.1720
1019 351.1794 350.1721
1020 351.1796 350.1723
1021 351.1797 350.1724
1022 351.1798 350.1725
1023 351.1803 350.1730
1024 351.1803 350.1730
1025 351.1804 350.1731
1026 351.1804 350.1731
1027 351.1808 350.1735
1028 351.1809 350.1736
1029 351.1810 350.1737
1030 351.1810 350.1737
1031 351.1812 350.1739
1032 351.1812 350.1739
1033 351.1814 350.1741
1034 351.1815 350.1742
1035 351.1984 350.1911
1036 351.1990 350.1917
1037 351.1992 350.1919
1038 351.1996 350.1923
1039 351.2003 350.1930
1040 351.2037 350.1964
1041 351.2154 350.2081
1042 351.2155 350.2082
1043 351.2163 350.2090
1044 351.2166 350.2093
1045 351.2168 350.2095
1046 351.2169 350.2096
1047 351.2170 350.2097
1048 351.2171 350.2098
1049 351.2172 350.2099
1050 351.2173 350.2100
1051 351.2175 350.2102
1052 351.2175 350.2102
1053 351.2177 350.2104
1054 351.2371 350.2298
1055 351.2374 350.2301
1056 351.2377 350.2304
1057 351.2378 350.2305
1058 351.2378 350.2305
1059 351.2379 350.2306
1060 351.2380 350.2307
1061 351.2383 350.2310
1062 352.0256 351.0183
1063 352.9712 351.9639
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