MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
864 341.1590 340.1517
865 341.1592 340.1519
866 341.1596 340.1523
867 341.1943 340.1870
868 341.1972 340.1899
869 341.1978 340.1905
870 341.2169 340.2096
871 342.0403 341.0330
872 342.0405 341.0332
873 342.0406 341.0333
874 342.0414 341.0341
875 342.0416 341.0343
876 342.0418 341.0345
877 342.0421 341.0348
878 342.0767 341.0694
879 342.1923 341.1850
880 342.1925 341.1852
881 342.1927 341.1854
882 342.3026 341.2953
883 343.0493 342.0420
884 343.0703 342.0630
885 343.1399 342.1326
886 343.2345 342.2272
887 344.0574 343.0501
888 344.0575 343.0502
889 344.2074 343.2001
890 344.2075 343.2002
891 344.2076 343.2003
892 344.2078 343.2005
893 344.2082 343.2009
894 344.2083 343.2010
895 344.2085 343.2012
896 344.2086 343.2013
897 344.2088 343.2015
898 344.2093 343.2020
899 344.2459 343.2386
900 344.2460 343.2387
901 345.0496 344.0423
902 345.0848 344.0775
903 345.1328 344.1255
904 345.2137 344.2064
905 345.2251 344.2178
906 345.2256 344.2183
907 346.0367 345.0294
908 346.2230 345.2157
909 346.2237 345.2164
910 346.2238 345.2165
911 346.2239 345.2166
912 346.2241 345.2168
913 346.2241 345.2168
914 346.2242 345.2169
915 346.2242 345.2169
916 346.2244 345.2171
917 346.2247 345.2174
918 347.0650 346.0577
919 347.1474 346.1401
920 347.1475 346.1402
921 347.1477 346.1404
922 347.1479 346.1406
923 347.1483 346.1410
924 347.1492 346.1419
925 347.1494 346.1421
926 347.1495 346.1422
927 347.1496 346.1423
928 347.1498 346.1425
929 347.1837 346.1764
930 347.1839 346.1766
931 347.1841 346.1768
932 347.1841 346.1768
933 347.1847 346.1774
934 347.1854 346.1781
935 347.1855 346.1782
936 347.1855 346.1782
937 347.1856 346.1783
938 347.1857 346.1784
939 347.1857 346.1784
940 347.1858 346.1785
941 347.1859 346.1786
942 347.1859 346.1786
943 347.1860 346.1787
944 347.1860 346.1787
945 347.1862 346.1789
946 347.1863 346.1790
947 347.1865 346.1792
948 347.1937 346.1864
949 348.2029 347.1956
950 348.2032 347.1959
951 349.1269 348.1196
952 349.1270 348.1197
953 349.1273 348.1200
954 349.1277 348.1204
955 349.1629 348.1556
956 349.1630 348.1557
957 349.1634 348.1561
958 349.1635 348.1562
959 349.1636 348.1563
960 349.1638 348.1565
961 349.1638 348.1565
962 349.1642 348.1569
963 349.1646 348.1573
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