MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
764 337.1270 336.1197
765 337.1271 336.1198
766 337.1272 336.1199
767 337.1275 336.1202
768 337.1282 336.1209
769 337.1288 336.1215
770 337.1288 336.1215
771 337.1305 336.1232
772 337.1626 336.1553
773 337.1630 336.1557
774 337.1631 336.1558
775 337.1634 336.1561
776 337.1634 336.1561
777 337.1634 336.1561
778 337.1636 336.1563
779 337.1638 336.1565
780 337.1640 336.1567
781 337.1641 336.1568
782 337.1641 336.1568
783 337.1646 336.1573
784 337.1646 336.1573
785 337.1646 336.1573
786 337.1647 336.1574
787 337.1649 336.1576
788 337.1654 336.1581
789 337.1655 336.1582
790 337.1659 336.1586
791 337.1660 336.1587
792 337.1825 336.1752
793 337.1833 336.1760
794 337.1836 336.1763
795 337.1836 336.1763
796 337.1836 336.1763
797 337.1837 336.1764
798 337.1839 336.1766
799 337.1844 336.1771
800 337.2385 336.2312
801 337.2574 336.2501
802 337.2579 336.2506
803 337.2583 336.2510
804 337.8462 336.8389
805 338.2403 337.2330
806 338.9880 337.9807
807 339.0764 338.0691
808 339.0767 338.0694
809 339.0770 338.0697
810 339.1048 338.0975
811 339.1064 338.0991
812 339.1064 338.0991
813 339.1065 338.0992
814 339.1075 338.1002
815 339.1139 338.1066
816 339.1422 338.1349
817 339.1422 338.1349
818 339.1423 338.1350
819 339.1424 338.1351
820 339.1424 338.1351
821 339.1426 338.1353
822 339.1428 338.1355
823 339.1428 338.1355
824 339.1429 338.1356
825 339.1433 338.1360
826 339.1437 338.1364
827 339.1440 338.1367
828 339.1789 338.1716
829 339.1791 338.1718
830 339.1794 338.1721
831 339.1795 338.1722
832 339.1796 338.1723
833 339.1800 338.1727
834 339.1806 338.1733
835 339.1813 338.1740
836 339.1813 338.1740
837 339.1817 338.1744
838 339.1819 338.1746
839 339.1982 338.1909
840 339.1992 338.1919
841 339.1993 338.1920
842 339.2002 338.1929
843 339.2007 338.1934
844 340.0255 339.0182
845 340.0618 339.0545
846 340.9830 339.9757
847 341.0555 340.0482
848 341.0684 340.0611
849 341.0921 340.0848
850 341.0926 340.0853
851 341.1207 340.1134
852 341.1208 340.1135
853 341.1216 340.1143
854 341.1218 340.1145
855 341.1232 340.1159
856 341.1574 340.1501
857 341.1577 340.1504
858 341.1580 340.1507
859 341.1581 340.1508
860 341.1583 340.1510
861 341.1584 340.1511
862 341.1586 340.1513
863 341.1588 340.1515
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