MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 4077)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
664 333.1701 332.1628
665 333.1701 332.1628
666 333.1706 332.1633
667 333.1708 332.1635
668 333.1708 332.1635
669 333.1896 332.1823
670 333.2045 332.1972
671 333.2049 332.1976
672 333.2055 332.1982
673 333.2057 332.1984
674 333.2058 332.1985
675 333.2058 332.1985
676 333.2060 332.1987
677 333.2061 332.1988
678 333.2061 332.1988
679 333.2062 332.1989
680 333.2062 332.1989
681 333.2063 332.1990
682 333.2063 332.1990
683 333.2064 332.1991
684 333.2066 332.1993
685 333.2067 332.1994
686 333.2071 332.1998
687 333.2242 332.2169
688 333.2247 332.2174
689 333.2271 332.2198
690 333.2272 332.2199
691 333.2277 332.2204
692 333.9637 332.9564
693 334.0106 333.0033
694 334.1874 333.1801
695 334.1875 333.1802
696 334.2227 333.2154
697 334.2233 333.2160
698 334.2242 333.2169
699 334.9594 333.9521
700 335.0823 334.0750
701 335.1003 334.0930
702 335.1110 334.1037
703 335.1111 334.1038
704 335.1111 334.1038
705 335.1113 334.1040
706 335.1114 334.1041
707 335.1118 334.1045
708 335.1169 334.1096
709 335.1472 334.1399
710 335.1477 334.1404
711 335.1478 334.1405
712 335.1481 334.1408
713 335.1482 334.1409
714 335.1483 334.1410
715 335.1483 334.1410
716 335.1483 334.1410
717 335.1485 334.1412
718 335.1487 334.1414
719 335.1490 334.1417
720 335.1492 334.1419
721 335.1494 334.1421
722 335.1497 334.1424
723 335.1841 334.1768
724 335.1844 334.1771
725 335.1852 334.1779
726 335.1854 334.1781
727 335.1855 334.1782
728 335.1858 334.1785
729 335.1858 334.1785
730 335.1858 334.1785
731 335.1858 334.1785
732 335.1859 334.1786
733 335.1859 334.1786
734 335.1859 334.1786
735 335.1860 334.1787
736 335.1861 334.1788
737 335.1862 334.1789
738 335.1866 334.1793
739 335.2209 334.2136
740 335.2209 334.2136
741 335.2212 334.2139
742 335.2215 334.2142
743 335.2216 334.2143
744 335.2217 334.2144
745 335.2219 334.2146
746 335.2219 334.2146
747 335.2220 334.2147
748 335.2221 334.2148
749 335.2221 334.2148
750 335.2222 334.2149
751 335.2222 334.2149
752 335.2224 334.2151
753 335.2226 334.2153
754 335.2408 334.2335
755 335.2412 334.2339
756 335.9669 334.9596
757 335.9796 334.9723
758 336.2258 335.2185
759 337.0977 336.0904
760 337.1257 336.1184
761 337.1264 336.1191
762 337.1266 336.1193
763 337.1269 336.1196
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