MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Unassigned peptides, 1001–1100 (out of 4077)

Page: Previous 1 …6 7 8 9 10 11 12 13 14 15 16 … 41 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
564 326.1978 325.1905
565 326.1982 325.1909
566 327.0759 326.0686
567 327.0764 326.0691
568 327.1050 326.0977
569 327.1057 326.0984
570 327.1066 326.0993
571 327.1069 326.0996
572 327.1071 326.0998
573 327.1426 326.1353
574 327.1429 326.1356
575 327.1434 326.1361
576 327.1435 326.1362
577 327.1436 326.1363
578 327.1436 326.1363
579 327.1439 326.1366
580 327.1439 326.1366
581 327.1443 326.1370
582 327.1446 326.1373
583 327.1451 326.1378
584 327.1793 326.1720
585 327.1989 326.1916
586 327.2002 326.1929
587 327.2005 326.1932
588 327.2010 326.1937
589 327.2174 326.2101
590 327.2367 326.2294
591 328.0175 327.0102
592 328.0265 327.0192
593 328.0485 327.0412
594 328.0616 327.0543
595 328.0617 327.0544
596 328.1470 327.1397
597 328.2128 327.2055
598 328.2141 327.2068
599 328.2142 327.2069
600 328.2144 327.2071
601 328.2338 327.2265
602 329.0549 328.0476
603 329.1237 328.1164
604 329.1584 328.1511
605 329.1587 328.1514
606 329.1588 328.1515
607 329.1590 328.1517
608 329.1591 328.1518
609 329.1592 328.1519
610 329.1593 328.1520
611 329.1597 328.1524
612 329.1598 328.1525
613 329.1951 328.1878
614 330.0401 329.0328
615 330.0403 329.0330
616 330.0408 329.0335
617 330.0414 329.0341
618 330.0417 329.0344
619 330.0765 329.0692
620 330.1671 329.1598
621 330.1914 329.1841
622 330.1921 329.1848
623 330.1922 329.1849
624 330.1926 329.1853
625 330.1931 329.1858
626 330.2301 329.2228
627 330.2303 329.2230
628 331.0488 330.0415
629 331.0850 330.0777
630 331.1163 330.1090
631 331.1766 330.1693
632 331.1767 330.1694
633 331.1899 330.1826
634 331.1906 330.1833
635 331.1907 330.1834
636 331.1912 330.1839
637 331.2108 330.2035
638 331.8875 330.8802
639 332.0563 331.0490
640 332.2075 331.2002
641 332.2076 331.2003
642 332.2077 331.2004
643 332.2079 331.2006
644 332.2080 331.2007
645 332.2081 331.2008
646 332.2089 331.2016
647 332.2092 331.2019
648 333.0569 332.0496
649 333.1316 332.1243
650 333.1324 332.1251
651 333.1324 332.1251
652 333.1325 332.1252
653 333.1330 332.1257
654 333.1333 332.1260
655 333.1685 332.1612
656 333.1694 332.1621
657 333.1696 332.1623
658 333.1698 332.1625
659 333.1699 332.1626
660 333.1700 332.1627
661 333.1700 332.1627
662 333.1701 332.1628
663 333.1701 332.1628
Page: Previous 1 …6 7 8 9 10 11 12 13 14 15 16 … 41 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/