MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 1 |
| MS data file | : | PRT1258_JBEI_Tube1_A1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:33:55 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,568 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_ECOLI | 7857 | Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RHAA_ECOLI | 967 | L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODP2_ECOLI | 915 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Escherichia coli (strain K12) GN=aceF PE=1 SV=3 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TKT1_ECOLI | 376 | Transketolase 1 OS=Escherichia coli (strain K12) GN=tktA PE=1 SV=5 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_ECOLI | 289 | Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 261 | Trypsin OS=Sus scrofa PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 6.1 | TRYP_PIG |
261 | 25078 | 12 (8) | 4 (4) | 0.87 | |
| Trypsin OS=Sus scrofa PE=1 SV=1 | |||||||
12 peptide matches (9 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2389 |
842.5120 | 841.5047 | 841.5022 | 3.06 | 0 | 6 | 0.63 | 1Score > 16 indicates identity |
U | R.VATVSLPR.S | |
| 2391 |
1 |
421.7609 | 841.5072 | 841.5022 | 6.06 | 0 | 53 | 1e-005 | 1Score > 16 indicates identity |
U | R.VATVSLPR.S |
| 2568 |
1 |
523.2879 | 1044.5612 | 1044.5564 | 4.69 | 0 | 54 | 2.4e-005 | 1Score > 20 indicates identity |
U | K.LSSPATLNSR.V |
| 3515 |
738.0422 | 2211.1048 | 2211.0807 | 10.9 | 0 | 72 | 2.5e-007 | 1Score > 19 indicates identity |
U | R.LGEHNIDVLEGNEQFINAAK.I + Deamidated (NQ) | |
| 3516 |
738.0444 | 2211.1114 | 2211.0807 | 13.9 | 0 | 49 | 5.7e-005 | 1Score > 19 indicates identity |
U | R.LGEHNIDVLEGNEQFINAAK.I + Deamidated (NQ) | |
| 3557 |
762.3923 | 2284.1551 | 2284.1409 | 6.20 | 0 | 2 | 2.6 | 1Score > 19 indicates identity |
U | K.IITHPNFNGNTLDNDIMLIK.L + 2 Deamidated (NQ) | |
| 3559 |
1 |
762.4016 | 2284.1830 | 2284.1409 | 18.4 | 0 | 41 | 0.00025 | 1Score > 18 indicates identity |
U | K.IITHPNFNGNTLDNDIMLIK.L + 2 Deamidated (NQ) |
| 3578 |
767.0671 | 2298.1795 | 2298.1678 | 5.08 | 0 | 8 | 0.82 | 1Score > 19 indicates identity |
U | K.IITHPNFNGNTLDNDIMLIK.L + Oxidation (M) | |
| 3581 |
767.7317 | 2300.1733 | 2300.1358 | 16.3 | 0 | 22 | 0.028 | 1Score > 19 indicates identity |
U | K.IITHPNFNGNTLDNDIMLIK.L + 2 Deamidated (NQ); Oxidation (M) |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CATNterm_ECOLX | 110 | Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | XYLB_ECOLI | 81 | Xylulose kinase OS=Escherichia coli (strain K12) GN=xylB PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FABZ_ECOLI | 73 | 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS=Escherichia coli (strain K12) GN=fabZ PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_ECOLI | 69 | 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2 |
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