MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 1 |
| MS data file | : | PRT1258_JBEI_Tube1_A1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:33:55 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,568 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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41| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LIGB_ECOLI | 18 | DNA ligase B OS=Escherichia coli (strain K12) GN=ligB PE=1 SV=2 |
42| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YBFC_ECOLI | 18 | Uncharacterized protein YbfC OS=Escherichia coli (strain K12) GN=ybfC PE=4 SV=1 |
43| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HUMS_ARATH | 17 | description |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 43.1 | HUMS_ARATH |
17 | 0 | 2 (1) | 1 (1) | 0.05 | |
| description | |||||||
2 peptide matches (1 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2223 |
1 |
627.3173 | 626.3100 | 626.3064 | 5.77 | 0 | 17 | 0.023 | 1Score > 13 indicates identity |
U | R.QFGFK.L + Deamidated (NQ) |
44| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NIKA_ECOLI | 15 | Nickel-binding periplasmic protein OS=Escherichia coli (strain K12) GN=nikA PE=1 SV=1 |
45| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FABF_ECOLI | 14 | 3-oxoacyl-[acyl-carrier-protein] synthase 2 OS=Escherichia coli (strain K12) GN=fabF PE=1 SV=2 |
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