MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 1 |
| MS data file | : | PRT1258_JBEI_Tube1_A1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:33:55 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,568 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_ECOLI | 7857 | Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RHAA_ECOLI | 967 | L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODP2_ECOLI | 915 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Escherichia coli (strain K12) GN=aceF PE=1 SV=3 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TKT1_ECOLI | 376 | Transketolase 1 OS=Escherichia coli (strain K12) GN=tktA PE=1 SV=5 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 4.1 | TKT1_ECOLI |
376 | 72451 | 21 (14) | 20 (14) | 0.86 | |
| Transketolase 1 OS=Escherichia coli (strain K12) GN=tktA PE=1 SV=5 | |||||||
21 peptide matches (21 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 2219 |
312.1618 | 622.3090 | 622.3075 | 2.54 | 0 | 5 | 1.1 | 2Score > 18 indicates identity |
U | K.YGVER.Q | |
| 2226 |
318.6901 | 635.3656 | 635.3643 | 2.19 | 0 | 28 | 0.0019 | 1Score > 13 indicates identity |
U | R.GGYVLK.D | |
| 2248 |
335.6820 | 669.3494 | 669.3486 | 1.29 | 0 | 23 | 0.0062 | 1Score > 13 indicates identity |
U | K.FAAYAK.A | |
D:\Xcalibur\Data\Jennifer\PRT1258 ThomasE\PRT1258_JBEI_Tube1_A1.rawScore > 17 indicates identity |
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| 2250 |
336.7014 | 671.3882 | 671.3854 | 4.28 | 0 | 16 | 0.079 | 1Score > 17 indicates identity |
U | R.ESVLPK.A | |
| -12.5 | 0 | 3 | 1.7 | 2 | TPVVTR | ||||||
| 2276 |
359.2118 | 716.4090 | 716.4068 | 3.08 | 0 | 34 | 0.002 | 1Score > 20 indicates identity |
U | K.LTAEGVK.A | |
| 2302 |
371.2173 | 740.4200 | 740.4181 | 2.68 | 0 | 11 | 0.11 | 1Score > 14 indicates identity |
U | K.LQANPAK.I | |
| 2345 |
393.7291 | 785.4436 | 785.4395 | 5.26 | 0 | 13 | 0.22 | 1Score > 19 indicates identity |
U | K.ELANAIR.A | |
| 2495 |
489.7516 | 977.4886 | 977.4852 | 3.56 | 0 | 34 | 0.0019 | 1Score > 19 indicates identity |
U | R.ALSMDAVQK.A + Oxidation (M) | |
| 2509 |
493.7567 | 985.4988 | 985.4941 | 4.84 | 0 | 15 | 0.047 | 1Score > 14 indicates identity |
U | R.QNLAQQER.T | |
| 2560 |
517.2892 | 1032.5638 | 1032.5604 | 3.35 | 0 | 49 | 4.2e-005 | 1Score > 18 indicates identity |
U | K.TIIGFGSPNK.A | |
| 2632 |
572.8041 | 1143.5936 | 1143.5884 | 4.62 | 0 | 57 | 8.3e-006 | 1Score > 19 indicates identity |
U | R.TEEQLANIAR.G | |
| 2660 |
585.8313 | 1169.6480 | 1169.6404 | 6.52 | 0 | 86 | 9.1e-009 | 1Score > 18 indicates identity |
U | R.QDGPTALILSR.Q | |
| 2788 |
426.5547 | 1276.6423 | 1276.6353 | 5.47 | 0 | 47 | 0.0001 | 1Score > 19 indicates identity |
U | R.FEAYGWHVIR.D | |
| 2790 |
641.8091 | 1281.6036 | 1281.5989 | 3.68 | 0 | 36 | 0.00083 | 1Score > 18 indicates identity |
U | K.AYPQEAAEFTR.R | |
| 2805 |
433.2222 | 1296.6448 | 1296.6422 | 1.97 | 1 | 50 | 2.9e-005 | 1Score > 17 indicates identity |
U | R.DIDGHDAASIKR.A | |
| 2806 |
325.1694 | 1296.6485 | 1296.6422 | 4.85 | 1 | 8 | 0.49 | 1Score > 17 indicates identity |
U | R.DIDGHDAASIKR.A | |
| 3258 |
588.2908 | 1761.8506 | 1761.8434 | 4.06 | 0 | 80 | 4.8e-008 | 1Score > 20 indicates identity |
U | K.AINEDAAGNYIHYGVR.E | |
| 3364 |
498.0021 | 1987.9793 | 1987.9712 | 4.09 | 0 | 36 | 0.0011 | 1Score > 19 indicates identity |
U | K.AGTHDSHGAPLGDAEIALTR.E | |
| 3383 |
673.3206 | 2016.9400 | 2016.9099 | 14.9 | 1 | 27 | 0.0071 | 1Score > 18 indicates identity |
U | R.VVSMPSTDAFDKQDAAYR.E + Deamidated (NQ); Oxidation (M) | |
| 4117 |
795.8967 | 3179.5577 | 3179.5194 | 12.1 | 0 | 13 | 0.087 | 1Score > 20 indicates identity Score > 15 indicates homology |
U | R.QVMVYTHDSIGLGEDGPTHQPVEQVASLR.V + Deamidated (NQ); Oxidation (M) | |
| 4175 |
1138.2435 | 3411.7087 | 3411.6504 | 17.1 | 0 | 5 | 1 | 1Score > 18 indicates identity |
U | K.TPGHPEVGYTAGVETTTGPLGQGIANAVGMAIAEK.T + 2 Deamidated (NQ); Oxidation (M) | |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| TKT2_ECOLI |
46 | 73225 | 4.1 | ||
| Transketolase 2 OS=Escherichia coli (strain K12) GN=tktB PE=1 SV=1 | |||||
| A0A4P9UVR0_9GAMM |
34 | 73148 | 4.1 | ||
| Transketolase OS=Methylotuvimicrobium buryatense OX=95641 GN=tkt PE=3 SV=1 | |||||
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_ECOLI | 289 | Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 261 | Trypsin OS=Sus scrofa PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CATNterm_ECOLX | 110 | Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | XYLB_ECOLI | 81 | Xylulose kinase OS=Escherichia coli (strain K12) GN=xylB PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FABZ_ECOLI | 73 | 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS=Escherichia coli (strain K12) GN=fabZ PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_ECOLI | 69 | 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2 |
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