MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 45)

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+31

Accession Score Description
1 ENTC_ECOLI 26 Isochorismate synthase EntC OS=Escherichia coli (strain K12) GN=entC PE=1 SV=1

+32

Accession Score Description
1 SODF_ECOLI 25 Superoxide dismutase [Fe] OS=Escherichia coli (strain K12) GN=sodB PE=1 SV=2

+33

Accession Score Description
1 YGDH_ECOLI 23 description

+34

Accession Score Description
1 PAAC_ECOLI 21 1,2-phenylacetyl-CoA epoxidase, subunit C OS=Escherichia coli (strain K12) GN=paaC PE=1 SV=1

+35

Accession Score Description
1 GUAA_ECOLI 21 GMP synthase [glutamine-hydrolyzing] OS=Escherichia coli (strain K12) GN=guaA PE=1 SV=1

+36

Accession Score Description
1 NAGA_ECOLI 20 N-acetylglucosamine-6-phosphate deacetylase OS=Escherichia coli (strain K12) GN=nagA PE=1 SV=1

+37

Accession Score Description
1 MCP2_ECOLI 20 Methyl-accepting chemotaxis protein II OS=Escherichia coli (strain K12) GN=tar PE=1 SV=2

-38

Accession Score Description
1 PRUB680 20 description
Score Mass Matches Sequences emPAI
38.1 PRUB680 20 0 1 (1) 1 (1) 0.07
description

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2246   666.3471 665.3398 665.3384 2.10 0 20 0.038 +1Score > 18 indicates identity U R.QYIDK.N

+39

Accession Score Description
1 PERM_ECOLI 19 Putative permease PerM OS=Escherichia coli (strain K12) GN=perM PE=3 SV=1

+40

Accession Score Description
1 DHE3_BOVIN 18 Glutamate dehydrogenase 1, mitochondrial OS=Bos taurus GN=GLUD1 PE=1 SV=2
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