MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | 1 |
| MS data file | : | PRT1258_JBEI_Tube1_A1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 24 Jun 2025 at 18:33:55 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,568 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_ECOLI | 7857 | Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RHAA_ECOLI | 967 | L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODP2_ECOLI | 915 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Escherichia coli (strain K12) GN=aceF PE=1 SV=3 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TKT1_ECOLI | 376 | Transketolase 1 OS=Escherichia coli (strain K12) GN=tktA PE=1 SV=5 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AMPA_ECOLI | 289 | Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 261 | Trypsin OS=Sus scrofa PE=1 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CATNterm_ECOLX | 110 | Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 7.1 | CATNterm_ECOLX |
110 | 27452 | 6 (4) | 4 (3) | 0.58 | |
| Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1 | |||||||
| 2 samesets of CATNterm_ECOLX | |||||||
| CAT_ECOLX |
110 | 25931 | 6 (4) | 4 (3) | 0.62 | ||
| Chloramphenicol acetyltransferase OS=Escherichia coli GN=cat PE=1 SV=1 | |||||||
| CAT_SALTI |
110 | 25931 | 6 (4) | 4 (3) | 0.62 | ||
| Chloramphenicol acetyltransferase OS=Salmonella typhi OX=90370 GN=cat PE=3 SV=1 | |||||||
6 peptide matches (5 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2623 |
377.5201 | 1129.5385 | 1129.5338 | 4.10 | 0 | 40 | 0.00028 | 1Score > 17 indicates identity |
U | R.LMNAHPEFR.M + Oxidation (M) | |
| 2624 |
565.7773 | 1129.5400 | 1129.5338 | 5.49 | 0 | 40 | 0.00032 | 1Score > 17 indicates identity |
U | R.LMNAHPEFR.M + Oxidation (M) | |
| 2862 |
1 |
449.9279 | 1346.7619 | 1346.7499 | 8.88 | 0 | 44 | 5.5e-005 | 1Score > 14 indicates identity |
U | K.FYPAFIHILAR.L |
| 2863 |
449.9281 | 1346.7625 | 1346.7499 | 9.33 | 0 | (2) | 0.88 | 1Score > 14 indicates identity |
U | K.FYPAFIHILAR.L | |
| 3181 |
559.6216 | 1675.8430 | 1675.8318 | 6.65 | 0 | 37 | 0.00085 | 1Score > 20 indicates identity Score > 19 indicates homology |
U | K.ITGYTTVDISQWHR.K | |
| 3654 |
475.4498 | 2372.2126 | 2372.1882 | 10.3 | 0 | 6 | 1.1 | 1Score > 19 indicates identity |
U | K.VLMPLAIQVHHAVCDGFHVGR.M + Deamidated (NQ); Oxidation (M) |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | XYLB_ECOLI | 81 | Xylulose kinase OS=Escherichia coli (strain K12) GN=xylB PE=1 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FABZ_ECOLI | 73 | 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS=Escherichia coli (strain K12) GN=fabZ PE=1 SV=1 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_ECOLI | 69 | 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2 |
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