MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 1
MS data file : PRT1258_JBEI_Tube1_A1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 24 Jun 2025 at 18:33:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,568

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 18 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 45)

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+1

Accession Score Description
1 DNAK_ECOLI 7857 Chaperone protein DnaK OS=Escherichia coli (strain K12) GN=dnaK PE=1 SV=2

-2

Accession Score Description
1 RHAA_ECOLI 967 L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2
Score Mass Matches Sequences emPAI
2.1 RHAA_ECOLI 967 47455 36 (30) 23 (20) 4.36
L-rhamnose isomerase OS=Escherichia coli (strain K12) GN=rhaA PE=1 SV=2

-36 peptide matches (30 non-duplicate, 6 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2220   624.4098 623.4025 623.4006 3.03 0 27 0.0022 +1Score > 13 indicates identity U R.LIPGPK.R
2221   312.7086 623.4026 623.4006 3.23 0 27 0.002 +1Score > 13 indicates identity U R.LIPGPK.R
2277   359.6911 717.3676 717.3657 2.68 0 13 0.23 +1Score > 19 indicates identity U K.DITVDR.L
2364 -1 401.6990 801.3834 801.3770 8.10 0 25 0.0064 +1Score > 15 indicates identity U R.HDLFDR.V
2363   401.6973 801.3800 801.3770 3.86 0 (12) 0.07 +1Score > 13 indicates identity U R.HDLFDR.V
2450   466.7368 931.4590 931.4552 4.15 0 15 0.12 +1Score > 19 indicates identity U K.NWVEWAK.A
2461 +1 472.2792 942.5438 942.5386 5.61 0 65 1e-006 +1Score > 18 indicates identity U R.LALLEEQK.S
2465   475.2256 948.4366 948.4335 3.37 0 28 0.0032 +1Score > 16 indicates identity U R.ADLEQAMR.L + Oxidation (M)
2510 +1 493.7954 985.5762 985.5709 5.43 0 52 1.8e-005 +1Score > 17 indicates identity U R.IAAWVIGTR.N
2585   533.7655 1065.5164 1065.5091 6.93 0 59 4.4e-006 +1Score > 18 indicates identity U K.LEAAGDYTAR.L
2629   571.3042 1140.5938 1140.5927 0.97 0 16 0.076 +1Score > 17 indicates identity U R.DQIKPEHFK.N
2630   381.2054 1140.5944 1140.5927 1.43 0 22 0.017 +1Score > 17 indicates identity U R.DQIKPEHFK.N
2688   398.8766 1193.6080 1193.6040 3.31 1 64 1.3e-006 +1Score > 18 indicates identity U R.KLEAAGDYTAR.L
2689   597.8125 1193.6104 1193.6040 5.39 1 55 9.7e-006 +1Score > 18 indicates identity U R.KLEAAGDYTAR.L
2812 +1 650.8775 1299.7404 1299.7285 9.16 0 95 6.7e-010 +1Score > 16 indicates identity U R.LLAALDEVISEK.L
2877 +1 695.3778 1388.7410 1388.7300 7.98 0 101 2.9e-010 +1Score > 18 indicates identity U R.FAAVGIDVEEALR.Q
2916   709.3760 1416.7374 1416.7249 8.88 0 65 1.2e-006 +1Score > 19 indicates identity U M.TTQLEQAWELAK.Q
2930   477.9145 1430.7217 1430.7154 4.41 0 32 0.0033 +1Score > 20 indicates identity U K.LNPAHHIDAVESK.L + Deamidated (NQ)
2931   358.6877 1430.7217 1430.7154 4.43 0 7 0.28 +1Score > 20 indicates identity
Score > 14 indicates homology		 U K.LNPAHHIDAVESK.L + Deamidated (NQ)
3091   528.5880 1582.7422 1582.7376 2.90 0 63 1.2e-006 +1Score > 16 indicates identity U R.HDTPAGSEWLESVR.A
3109 +1 535.2838 1602.8296 1602.8154 8.81 0 65 1.6e-006 +1Score > 19 indicates identity U R.VHIGLDFFDASINR.I
3111   802.4229 1602.8312 1602.8154 9.86 0 33 0.0021 +1Score > 19 indicates identity U R.VHIGLDFFDASINR.I
3291   610.3277 1827.9613 1827.9366 13.5 0 51 2.8e-005 +1Score > 18 indicates identity U R.LNLHAIYLESDTPVSR.D + Deamidated (NQ)
3359   662.0286 1983.0640 1983.0537 5.16 1 52 1.8e-005 +1Score > 17 indicates identity U K.RLNLHAIYLESDTPVSR.D
3360   497.0239 1984.0665 1984.0378 14.5 1 25 0.0082 +1Score > 17 indicates identity U K.RLNLHAIYLESDTPVSR.D + Deamidated (NQ)
3361   662.3633 1984.0681 1984.0378 15.3 1 24 0.011 +1Score > 17 indicates identity U K.RLNLHAIYLESDTPVSR.D + Deamidated (NQ)
3529   560.7816 2239.0973 2239.0579 17.6 0 32 0.0029 +1Score > 19 indicates identity U R.QTALCLDAGHFHPTEVISDK.I + Deamidated (NQ)
3550   570.8380 2279.3229 2279.2824 17.8 0 16 0.027 +1Score > 13 indicates identity U K.ISAAMLYVPQLLLHVSRPVR.W + Deamidated (NQ); Oxidation (M)
3750   838.0913 2511.2521 2511.2129 15.6 0 24 0.022 +1Score > 20 indicates identity U R.WDSDHVVLLDDETQAIASEIVR.H + Deamidated (NQ)
3812   879.7432 2636.2078 2636.1741 12.8 0 48 6.1e-005 +1Score > 18 indicates identity U K.LFGIGAESYTVGSNEFYMGYATSR.Q + Deamidated (NQ); Oxidation (M)
3956   933.1246 2796.3520 2796.3615 -3.41 1 2 2.6 +3Score > 19 indicates identity U R.RVSAYFGEQLGTPSVMNIWIPDGMK.D + Deamidated (NQ)

+3

Accession Score Description
1 ODP2_ECOLI 915 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Escherichia coli (strain K12) GN=aceF PE=1 SV=3

+4

Accession Score Description
1 TKT1_ECOLI 376 Transketolase 1 OS=Escherichia coli (strain K12) GN=tktA PE=1 SV=5

+5

Accession Score Description
1 AMPA_ECOLI 289 Cytosol aminopeptidase OS=Escherichia coli (strain K12) GN=pepA PE=1 SV=1

+6

Accession Score Description
1 TRYP_PIG 261 Trypsin OS=Sus scrofa PE=1 SV=1

+7

Accession Score Description
1 CATNterm_ECOLX 110 Chloramphenicol acetyltransferase with N-terminal tag OS=Escherichia coli GN=cat PE=1 SV=1

+8

Accession Score Description
1 XYLB_ECOLI 81 Xylulose kinase OS=Escherichia coli (strain K12) GN=xylB PE=1 SV=1

+9

Accession Score Description
1 FABZ_ECOLI 73 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS=Escherichia coli (strain K12) GN=fabZ PE=1 SV=1

+10

Accession Score Description
1 CH60_ECOLI 69 60 kDa chaperonin OS=Escherichia coli (strain K12) GN=groL PE=1 SV=2
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