MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
396 321.1678 320.1605
397 321.1680 320.1607
398 321.1681 320.1608
399 321.1682 320.1609
400 321.1682 320.1609
401 321.1684 320.1611
402 321.1685 320.1612
403 321.1686 320.1613
404 321.1686 320.1613
405 321.1687 320.1614
406 321.1688 320.1615
407 321.1689 320.1616
408 321.1689 320.1616
409 321.1689 320.1616
410 321.1690 320.1617
411 321.1690 320.1617
412 321.1692 320.1619
413 321.1693 320.1620
414 321.1693 320.1620
415 321.1693 320.1620
416 321.2056 320.1983
417 321.2056 320.1983
418 321.2057 320.1984
419 322.1562 321.1489
420 323.0264 322.0191
421 323.1101 322.1028
422 323.1104 322.1031
423 323.1104 322.1031
424 323.1104 322.1031
425 323.1110 322.1037
426 323.1110 322.1037
427 323.1110 322.1037
428 323.1110 322.1037
429 323.1111 322.1038
430 323.1113 322.1040
431 323.1116 322.1043
432 323.1118 322.1045
433 323.1462 322.1389
434 323.1471 322.1398
435 323.1472 322.1399
436 323.1472 322.1399
437 323.1472 322.1399
438 323.1474 322.1401
439 323.1474 322.1401
440 323.1474 322.1401
441 323.1475 322.1402
442 323.1475 322.1402
443 323.1475 322.1402
444 323.1475 322.1402
445 323.1476 322.1403
446 323.1479 322.1406
447 323.1480 322.1407
448 323.1481 322.1408
449 323.1482 322.1409
450 323.1483 322.1410
451 323.1485 322.1412
452 323.1485 322.1412
453 323.1652 322.1579
454 323.1658 322.1585
455 323.1660 322.1587
456 323.1662 322.1589
457 323.1665 322.1592
458 323.1837 322.1764
459 323.1840 322.1767
460 323.1841 322.1768
461 323.1843 322.1770
462 323.1843 322.1770
463 323.1844 322.1771
464 323.1844 322.1771
465 323.1844 322.1771
466 323.1847 322.1774
467 323.1848 322.1775
468 323.1848 322.1775
469 323.1848 322.1775
470 323.1848 322.1775
471 323.1850 322.1777
472 323.1850 322.1777
473 323.1850 322.1777
474 323.1851 322.1778
475 325.0607 324.0534
476 325.0992 324.0919
477 325.1064 324.0991
478 325.1070 324.0997
479 325.1261 324.1188
480 325.1261 324.1188
481 325.1263 324.1190
482 325.1266 324.1193
483 325.1266 324.1193
484 325.1267 324.1194
485 325.1267 324.1194
486 325.1268 324.1195
487 325.1268 324.1195
488 325.1270 324.1197
489 325.1271 324.1198
490 325.1271 324.1198
491 325.1273 324.1200
492 325.1276 324.1203
493 325.1631 324.1558
494 325.1631 324.1558
495 325.1632 324.1559
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