MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : 2
MS data file : PRT1249__2.mgf
Database : Ecoli-MetEng 20200720 (4,898 sequences; 1,660,463 residues)
Timestamp : 23 Jun 2025 at 17:45:54 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,602

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 17 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 4272)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
96 305.1734 304.1661
97 305.1734 304.1661
98 305.1735 304.1662
99 305.1738 304.1665
100 305.1738 304.1665
101 305.1738 304.1665
102 305.1739 304.1666
103 305.1739 304.1666
104 305.1741 304.1668
105 305.1741 304.1668
106 305.1742 304.1669
107 305.1742 304.1669
108 305.1742 304.1669
109 305.1743 304.1670
110 305.1743 304.1670
111 306.1549 305.1476
112 306.1560 305.1487
113 306.1921 305.1848
114 306.1927 305.1854
115 307.0410 306.0337
116 307.0789 306.0716
117 307.0958 306.0885
118 307.1151 306.1078
119 307.1155 306.1082
120 307.1156 306.1083
121 307.1158 306.1085
122 307.1158 306.1085
123 307.1163 306.1090
124 307.1163 306.1090
125 307.1164 306.1091
126 307.1164 306.1091
127 307.1165 306.1092
128 307.1165 306.1092
129 307.1165 306.1092
130 307.1166 306.1093
131 307.1169 306.1096
132 307.1522 306.1449
133 307.1524 306.1451
134 307.1524 306.1451
135 307.1526 306.1453
136 307.1527 306.1454
137 307.1527 306.1454
138 307.1527 306.1454
139 307.1528 306.1455
140 307.1529 306.1456
141 307.1529 306.1456
142 307.1531 306.1458
143 307.1531 306.1458
144 307.1531 306.1458
145 307.1532 306.1459
146 307.1533 306.1460
147 307.1533 306.1460
148 307.1534 306.1461
149 307.1534 306.1461
150 307.1535 306.1462
151 307.1890 306.1817
152 307.1892 306.1819
153 307.1893 306.1820
154 307.1893 306.1820
155 307.1894 306.1821
156 307.1896 306.1823
157 307.1897 306.1824
158 307.1899 306.1826
159 307.1900 306.1827
160 307.1900 306.1827
161 307.1900 306.1827
162 307.1900 306.1827
163 307.1900 306.1827
164 307.1902 306.1829
165 307.1902 306.1829
166 307.1902 306.1829
167 309.0943 308.0870
168 309.0945 308.0872
169 309.0948 308.0875
170 309.0950 308.0877
171 309.0951 308.0878
172 309.0954 308.0881
173 309.1114 308.1041
174 309.1310 308.1237
175 309.1312 308.1239
176 309.1314 308.1241
177 309.1316 308.1243
178 309.1318 308.1245
179 309.1319 308.1246
180 309.1319 308.1246
181 309.1319 308.1246
182 309.1320 308.1247
183 309.1320 308.1247
184 309.1320 308.1247
185 309.1321 308.1248
186 309.1321 308.1248
187 309.1321 308.1248
188 309.1322 308.1249
189 309.1322 308.1249
190 309.1324 308.1251
191 309.1324 308.1251
192 309.1325 308.1252
193 309.1512 308.1439
194 309.1677 308.1604
195 309.1677 308.1604
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